Hydrolysis of GTP by p21NRAS, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: use of a single fluorescent probe at the catalytic site
about
Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysisCharacterization of the interactions between the small GTPase Cdc42 and its GTPase-activating proteins and putative effectors. Comparison of kinetic properties of Cdc42 binding to the Cdc42-interactive domainsFluorescent coumarin-labeled nucleotides to measure ADP release from actomyosinReal-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues.Analysis of guanine nucleotide binding and exchange kinetics of the Escherichia coli GTPase Era.The Caulobacter crescentus CgtA protein displays unusual guanine nucleotide binding and exchange properties.Functional reconstitution of an atypical G protein heterotrimer and regulator of G protein signaling protein (RGS1) from Arabidopsis thaliana.Kinetic isotope effects in Ras-catalyzed GTP hydrolysis: evidence for a loose transition state.Nucleotide binding by the erythrocyte transglutaminase/Gh protein, probed with fluorescent analogs of GTP and GDP.Fluorescence detection of GDP in real time with the reagentless biosensor rhodamine-ParM.Relevance of the kinetic equilibrium of forces to the control of the cell cycle by Ras proteins.Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled gamma-phosphate-linked GTP analogs.The Escherichia coli GTPase CgtAE cofractionates with the 50S ribosomal subunit and interacts with SpoT, a ppGpp synthetase/hydrolase.The biochemistry of ras p21.The importance of two conserved arginine residues for catalysis by the ras GTPase-activating protein, neurofibromin.Kinetics of interaction of Rab5 and Rab7 with nucleotides and magnesium ions.The small GTPases K-Ras, N-Ras, and H-Ras have distinct biochemical properties determined by allosteric effects.Kinetic timing: a novel mechanism that improves the accuracy of GTPase timers in endosome fusion and other biological processes.Farnesyltransferase as a target for anticancer drug design.Interaction of GTPase-activating protein with p21ras, measured using a continuous assay for inorganic phosphate release.Role of the switch II region in the conformational transition of activation of Ha-ras-p21.Characterization of the hinges of the effector loop in the reaction pathway of the activation of ras-proteins. Kinetics of binding of beryllium trifluoride to V29G and I36G mutants of Ha-ras-p21.Nitrogenase of Klebsiella pneumoniae. Reversibility of the reductant-independent MgATP-cleavage reaction is shown by MgADP-catalysed phosphate/water oxygen exchange.Real-time NMR study of three small GTPases reveals that fluorescent 2'(3')-O-(N-methylanthraniloyl)-tagged nucleotides alter hydrolysis and exchange kinetics.Rac1, a low-molecular-mass GTP-binding-protein with high intrinsic GTPase activity and distinct biochemical properties.The trifunctional sulfate-activating complex (SAC) of Mycobacterium tuberculosis.Ras proteins increase Ca(2+)-responsiveness of smooth muscle contraction.Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins.Biochemical studies of the mechanism of action of the Cdc42-GTPase-activating protein.Mechanism of inhibition by arachidonic acid of the catalytic activity of Ras GTPase-activating proteins.
P2860
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P2860
Hydrolysis of GTP by p21NRAS, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: use of a single fluorescent probe at the catalytic site
description
1990 nî lūn-bûn
@nan
1990 թուականի Մայիսին հրատարակուած գիտական յօդուած
@hyw
1990 թվականի մայիսին հրատարակված գիտական հոդված
@hy
1990年の論文
@ja
1990年論文
@yue
1990年論文
@zh-hant
1990年論文
@zh-hk
1990年論文
@zh-mo
1990年論文
@zh-tw
1990年论文
@wuu
name
Hydrolysis of GTP by p21NRAS, ...... nt probe at the catalytic site
@ast
Hydrolysis of GTP by p21NRAS, ...... nt probe at the catalytic site
@en
Hydrolysis of GTP by p21NRAS, ...... t probe at the catalytic site.
@nl
type
label
Hydrolysis of GTP by p21NRAS, ...... nt probe at the catalytic site
@ast
Hydrolysis of GTP by p21NRAS, ...... nt probe at the catalytic site
@en
Hydrolysis of GTP by p21NRAS, ...... t probe at the catalytic site.
@nl
prefLabel
Hydrolysis of GTP by p21NRAS, ...... nt probe at the catalytic site
@ast
Hydrolysis of GTP by p21NRAS, ...... nt probe at the catalytic site
@en
Hydrolysis of GTP by p21NRAS, ...... t probe at the catalytic site.
@nl
P2093
P2860
P356
P1476
Hydrolysis of GTP by p21NRAS, ...... nt probe at the catalytic site
@en
P2093
P2860
P304
P356
10.1073/PNAS.87.9.3562
P407
P577
1990-05-01T00:00:00Z