Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins.
about
Structural insights into the antigenicity of myelin oligodendrocyte glycoproteinImproved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding siteCrystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop regionImpaired surface membrane insertion of homo- and heterodimeric human muscle chloride channels carrying amino-terminal myotonia-causing mutationsLactococcus lactis YfiA is necessary and sufficient for ribosome dimerizationSoluble fusion proteins between single transmembrane photoreceptor guanylyl cyclases and their activators.The cysteine dioxygenase homologue from Pseudomonas aeruginosa is a 3-mercaptopropionate dioxygenasePlasmid vectors for proteomic analyses in Giardia: purification of virulence factors and analysis of the proteasomeIdentification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicumThe AraC-type regulator RipA represses aconitase and other iron proteins from Corynebacterium under iron limitation and is itself repressed by DtxRThe 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligandsThe transcriptional activator ClgR controls transcription of genes involved in proteolysis and DNA repair in Corynebacterium glutamicum.Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D.Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.Dke1--structure, dynamics, and function: a theoretical and experimental study elucidating the role of the binding site shape and the hydrogen-bonding network in catalysis.Analysis of ER resident proteins in Saccharomyces cerevisiae: implementation of H/KDEL retrieval sequences.Duocalins: engineered ligand-binding proteins with dual specificity derived from the lipocalin fold.Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins.Identification of the tRNA-binding protein Arc1p as a novel target of in vivo biotinylation in Saccharomyces cerevisiae.Application of the Fc fusion format to generate tag-free bi-specific diabodies.Mutagenesis of Klebsiella aerogenes UreG to probe nickel binding and interactions with other urease-related proteins.A Protein Epitope Signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines.Inter-allelic prion propagation reveals conformational relationships among a multitude of [PSI] strains.Assembly of CS1 pili: the role of specific residues of the major pilin, CooAHepatoma-derived growth factor and nucleolin exist in the same ribonucleoprotein complex.Construction of a synthetic phage-displayed Nanobody library with CDR3 regions randomized by trinucleotide cassettes for diagnostic applications.Characterization of norovirus RNA replicase for in vitro amplification of RNACrystal structures of fusion proteins with large-affinity tags.Site-directed mutagenesis maps interactions that enhance cognate and limit promiscuous catalysis by an alkaline phosphatase superfamily phosphodiesterase.Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol CouplingMonkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunitIdentification of RNA helicase A as a cellular factor that interacts with influenza A virus NS1 protein and its role in the virus life cycleAmino Acid Proximities in Two Sup35 Prion Strains Revealed by Chemical Cross-linking.W8, a new Sup35 prion strain, transmits distinctive information with a conserved assembly scheme.Modular broad-host-range expression vectors for single-protein and protein complex purification.Engineered single-chain, antiparallel, coiled coil mimics the MerR metal binding siteStrain-specific sequences required for yeast [PSI+] prion propagation.Overview of affinity tags for protein purification.Peptide-based Biopolymers in Biomedicine and Biotechnology.Exploiting features of adenovirus replication to support mammalian kinase production
P2860
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P2860
Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins.
description
2000 nî lūn-bûn
@nan
2000 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
2000 թվականի հունվարին հրատարակված գիտական հոդված
@hy
2000年の論文
@ja
2000年論文
@yue
2000年論文
@zh-hant
2000年論文
@zh-hk
2000年論文
@zh-mo
2000年論文
@zh-tw
2000年论文
@wuu
name
Use of the Strep-Tag and strep ...... ation of recombinant proteins.
@ast
Use of the Strep-Tag and strep ...... ation of recombinant proteins.
@en
type
label
Use of the Strep-Tag and strep ...... ation of recombinant proteins.
@ast
Use of the Strep-Tag and strep ...... ation of recombinant proteins.
@en
prefLabel
Use of the Strep-Tag and strep ...... ation of recombinant proteins.
@ast
Use of the Strep-Tag and strep ...... ation of recombinant proteins.
@en
P1476
Use of the Strep-Tag and strep ...... ation of recombinant proteins.
@en
P2093
P304
P356
10.1016/S0076-6879(00)26060-6
P407
P577
2000-01-01T00:00:00Z