Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
about
Engineering of temperature- and light-switchable Cas9 variantsEngineering extrinsic disorder to control protein activity in living cells.Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation.Genetic and epigenetic control of gene expression by CRISPR-Cas systems.Multidimensional chemical control of CRISPR-Cas9.Zebrafish Genome Engineering Using the CRISPR-Cas9 System.New CRISPR-Cas systems from uncultivated microbes.Rapid construction of metabolite biosensors using domain-insertion profiling.Biofuel metabolic engineering with biosensors.High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding.Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells.Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies.Applications of CRISPR genome editing technology in drug target identification and validation.CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes.Cornerstones of CRISPR-Cas in drug discovery and therapy.Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors.Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors.Inducible CRISPR genome-editing tool: classifications and future trends.Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains.Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing.Multidimensional Control of Cas9 by Evolved RNA Polymerase-Based Biosensors.Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells.RNA-dependent RNA targeting by CRISPR-Cas9.Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion.Harnessing natural DNA modifying activities for editing of the genome and epigenome.Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell Type Specific Gene Editing.Design principles for nuclease-deficient CRISPR-based transcriptional regulators.The CRISPR tool kit for genome editing and beyond.Protein Engineering Strategies to Expand CRISPR-Cas9 ApplicationsIncreasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair templateHIT-Cas9: A CRISPR/Cas9 Genome-Editing Device under Tight and Effective Drug Control
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P2860
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
description
2016 nî lūn-bûn
@nan
2016 թուականի Մայիսին հրատարակուած գիտական յօդուած
@hyw
2016 թվականի մայիսին հրատարակված գիտական հոդված
@hy
2016年の論文
@ja
2016年論文
@yue
2016年論文
@zh-hant
2016年論文
@zh-hk
2016年論文
@zh-mo
2016年論文
@zh-tw
2016年论文
@wuu
name
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@ast
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@en
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@nl
type
label
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@ast
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@en
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@nl
prefLabel
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@ast
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@en
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@nl
P2093
P2860
P50
P356
P1433
P1476
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
@en
P2093
Benjamin L Oakes
Brett T Staahl
David F Savage
P2860
P2888
P304
P356
10.1038/NBT.3528
P577
2016-05-02T00:00:00Z