General method for direct cloning of DNA fragments generated by the polymerase chain reaction.
about
Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damageMammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organellesEvolutionary conservation of excision repair in Schizosaccharomyces pombe: evidence for a family of sequences related to the Saccharomyces cerevisiae RAD2 geneMolecular and cellular analysis of the DNA repair defect in a patient in xeroderma pigmentosum complementation group D who has the clinical features of xeroderma pigmentosum and Cockayne syndromeNormal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain proteinExpression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces.Characterization of the five replication factor C genes of Saccharomyces cerevisiaeElucidation of a promoter activity that directs the expression of acetyl-CoA carboxylase alpha with an alternative N-terminus in a tissue-restricted fashion.Molecular and biochemical characterisation of DNA-dependent protein kinase-defective rodent mutant irs-20MiR-RACE, a new efficient approach to determine the precise sequences of computationally identified trifoliate orange (Poncirus trifoliata) microRNAs.Telomerase-dependent repeat divergence at the 3' ends of yeast telomeres.Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacerDevelopment of a recombinant nucleoprotein-based enzyme-linked immunosorbent assay for quantification of antibodies against porcine reproductive and respiratory syndrome virusEvidence of bluetongue virus serotype 21 (BTV-21) divergence.Use of quantitative competitive PCR to measure Epstein-Barr virus genome load in the peripheral blood of pediatric transplant patients with lymphoproliferative disorders.Systematic repeat addition at a precise location in the coding region of the involucrin gene of wild mice reveals their phylogeny.The U3 promoter region of the acutely lethal simian immunodeficiency virus clone smmPBj1.9 confers related biological activity on the apathogenic clone agm3mc.A series of TA-based and zero-background vectors for plant functional genomics.Origin of the polymorphism of the involucrin gene in AsiansEpstein-Barr virus latent messages with shuffled leader exons: remnants of circumgenomic transcription?Id3 prevents differentiation of preadipose cells.The rad16 gene of Schizosaccharomyces pombe: a homolog of the RAD1 gene of Saccharomyces cerevisiaeMating type protein Mat1-2 from asexual Aspergillus fumigatus drives sexual reproduction in fertile Aspergillus nidulans.Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouseTwo morbilliviruses implicated in bottlenose dolphin epizooticsSequences of genes encoding type-specific and group-specific antigens of an Indian isolate of bluetongue virus serotype 10 (BTV-10) and implications for their origin.Structure-function studies of the adenylate cyclase toxin of Bordetella pertussis and the leukotoxin of Pasteurella haemolytica by heterologous C protein activation and construction of hybrid proteinsThe genomic organization of the region containing the Drosophila melanogaster rpL7a (Surf-3) gene differs from those of the mammalian and avian Surfeit loci.Molecular and biochemical characterization of xrs mutants defective in Ku80.In vivo cloning of PCR products in E. coli.DISEC-TRISEC: di- and trinucleotide-sticky-end cloning of PCR-amplified DNANovel constructs for efficient cloning of sRNA-encoding DNA and uniform silencing of plant genes employing artificial trans-acting small interfering RNA.Hypomethylation of the Xmrk oncogene promoter in melanoma cells of Xiphophorus.Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+)An efficient method to prepare PCR cloning vectors.The microsomal epoxide hydrolase has a single membrane signal anchor sequence which is dispensable for the catalytic activity of this protein.Small-subunit rRNA processome proteins are translationally regulated during differentiation of Trypanosoma cruzi.Evidence that the N-terminal extension of the vicieae convicilin genes evolved by intragenic duplications and trinucleotide expansions.pSDTV vector: a modification of the pBluescript SK+ plasmid in order to perform PCR-fragments TA-cloning using Eam1105I restriction endonuclease.Genetic characterization of bluetongue virus serotype 9 isolates from India.
P2860
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P2860
General method for direct cloning of DNA fragments generated by the polymerase chain reaction.
description
1991 nî lūn-bûn
@nan
1991年の論文
@ja
1991年論文
@yue
1991年論文
@zh-hant
1991年論文
@zh-hk
1991年論文
@zh-mo
1991年論文
@zh-tw
1991年论文
@wuu
1991年论文
@zh
1991年论文
@zh-cn
name
General method for direct clon ...... the polymerase chain reaction.
@ast
General method for direct clon ...... the polymerase chain reaction.
@en
type
label
General method for direct clon ...... the polymerase chain reaction.
@ast
General method for direct clon ...... the polymerase chain reaction.
@en
prefLabel
General method for direct clon ...... the polymerase chain reaction.
@ast
General method for direct clon ...... the polymerase chain reaction.
@en
P2093
P2860
P356
P1476
General method for direct clon ...... the polymerase chain reaction.
@en
P2093
P2860
P356
10.1093/NAR/19.16.4560
P407
P577
1991-08-01T00:00:00Z