CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA
about
Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice.Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJEfficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 SystemUltra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice.TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus.Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote.Developmental history and application of CRISPR in human disease.A comprehensive overview of computational resources to aid in precision genome editing with engineered nucleases.CRISPR: a versatile tool for both forward and reverse genetics research.A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes.Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9.Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation.Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors.Magic wands of CRISPR-lots of choices for gene knock-in.High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0 Xenopus.Optimized co-transfection of murine embryonic stem cells.i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.Comparison of DNA fragments as donor DNAs upon sequence conversion of cleaved target DNA.CLICK: one-step generation of conditional knockout mice.Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles.Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants.
P2860
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P2860
CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA
description
2015 nî lūn-bûn
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2015年の論文
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2015年論文
@yue
2015年論文
@zh-hant
2015年論文
@zh-hk
2015年論文
@zh-mo
2015年論文
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2015年论文
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2015年论文
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2015年论文
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name
CRISPR/Cas9-based generation o ...... ing longer single-stranded DNA
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CRISPR/Cas9-based generation o ...... ing longer single-stranded DNA
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type
label
CRISPR/Cas9-based generation o ...... ing longer single-stranded DNA
@ast
CRISPR/Cas9-based generation o ...... ing longer single-stranded DNA
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prefLabel
CRISPR/Cas9-based generation o ...... ing longer single-stranded DNA
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CRISPR/Cas9-based generation o ...... ing longer single-stranded DNA
@en
P2093
P2860
P356
P1433
P1476
CRISPR/Cas9-based generation o ...... ing longer single-stranded DNA
@en
P2093
Channabasavaiah B Gurumurthy
Hiromi Miura
Masahiro Sato
Masato Ohtsuka
Takehito Sato
P2860
P2888
P356
10.1038/SREP12799
P407
P577
2015-08-05T00:00:00Z