Dissecting the multistep reaction pathway of an RNA enzyme by single-molecule kinetic "fingerprinting".
about
Single VS ribozyme molecules reveal dynamic and hierarchical folding toward catalysis.Synchronously amplified fluorescence image recovery (SAFIRe).Construction of a laser combiner for dual fluorescent single molecule imaging of pRNA of phi29 DNA packaging motor.Quantifying DNA melting transitions using single-molecule force spectroscopy.Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF.RNA reactions one molecule at a timeSingle-molecule tools for enzymology, structural biology, systems biology and nanotechnology: an update.Do-it-yourself guide: how to use the modern single-molecule toolkitDynamic motions of the HIV-1 frameshift site RNA.Single-molecule spectroscopy reveals how calmodulin activates NO synthase by controlling its conformational fluctuation dynamics.Mutational inhibition of ligation in the hairpin ribozyme: substitutions of conserved nucleobases A9 and A10 destabilize tertiary structure and selectively promote cleavage.Minimal and extended hammerheads utilize a similar dynamic reaction mechanism for catalysis.Intermolecular domain docking in the hairpin ribozyme: metal dependence, binding kinetics and catalysisRNA dynamics: it is about time.A rugged free energy landscape separates multiple functional RNA folds throughout denaturationKinetic analysis of sequential multistep reactionsQuantum mechanical/molecular mechanical simulation study of the mechanism of hairpin ribozyme catalysisMolecular dynamics suggest multifunctionality of an adenine imino group in acid-base catalysis of the hairpin ribozyme.Single-molecule observations of ribosome functionSingle molecule imaging approach to membrane protein stoichiometry.Transport dynamics in a glutamate transporter homologue.Domain-elongation NMR spectroscopy yields new insights into RNA dynamics and adaptive recognition.Sizing up single-molecule enzymatic conformational dynamics.Nanoscopic optical rulers beyond the FRET distance limit: fundamentals and applications.Engineering of ribozymes with useful activities in the ancient RNA world.Characterizing excited conformational states of RNA by NMR spectroscopyThermodynamics and kinetics of RNA tertiary structure formation in the junctionless hairpin ribozyme.Probing single-molecule enzyme active-site conformational state intermittent coherence.Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function.Using stochastic models calibrated from nanosecond nonequilibrium simulations to approximate mesoscale information.Single-molecule FRET TACKLE reveals highly dynamic mismatched DNA-MutS complexes.Sequence-controlled RNA self-processing: computational design, biochemical analysis, and visualization by AFM.Analysis of complex single-molecule FRET time trajectories.Analyzing single-molecule manipulation experiments.Quantifying multiscale noise sources in single-molecule time series.Oligonucleotide-stabilized Ag nanocluster fluorophoresNative purification and labeling of RNA for single molecule fluorescence studiesRNA catalysis as a probe for chaperone activity of DEAD-box helicases.The shape-shifting quasispecies of RNA: one sequence, many functional foldsThermodynamics and kinetics of the hairpin ribozyme from atomistic folding/unfolding simulations.
P2860
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P2860
Dissecting the multistep reaction pathway of an RNA enzyme by single-molecule kinetic "fingerprinting".
description
2007 nî lūn-bûn
@nan
2007年の論文
@ja
2007年論文
@yue
2007年論文
@zh-hant
2007年論文
@zh-hk
2007年論文
@zh-mo
2007年論文
@zh-tw
2007年论文
@wuu
2007年论文
@zh
2007年论文
@zh-cn
name
Dissecting the multistep react ...... cule kinetic "fingerprinting".
@ast
Dissecting the multistep react ...... cule kinetic "fingerprinting".
@en
type
label
Dissecting the multistep react ...... cule kinetic "fingerprinting".
@ast
Dissecting the multistep react ...... cule kinetic "fingerprinting".
@en
prefLabel
Dissecting the multistep react ...... cule kinetic "fingerprinting".
@ast
Dissecting the multistep react ...... cule kinetic "fingerprinting".
@en
P2093
P2860
P356
P1476
Dissecting the multistep react ...... ecule kinetic "fingerprinting"
@en
P2093
Gregory Bokinsky
Shixin Liu
Xiaowei Zhuang
P2860
P304
12634-12639
P356
10.1073/PNAS.0610597104
P407
P577
2007-05-11T00:00:00Z