Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.
about
Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzymeAssays for the preferential binding of human topoisomerase I to supercoiled DNA.Isolation and characterization of conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae.Misincorporation during DNA synthesis, analyzed by gel electrophoresis.Site specific mutagenesis: insertion of single noncomplementary nucleotides at specified sites by error-directed DNA polymerizationA complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.Topological locking restrains replication fork reversal.Recombination between directly repeated origins of conjugative transfer cloned in M13 bacteriophage DNA models ligation of the transferred plasmid strand.Chirality of DNA trefoils: implications in intramolecular synapsis of distant DNA segments.Yeast carboxypeptidase Y can be translocated and glycosylated without its amino-terminal signal sequence.The ornithine transcarbamylase leader peptide directs mitochondrial import through both its midportion structure and net positive charge.Diverse effects of beta-tubulin mutations on microtubule formation and functionEnzymatic techniques for the isolation of random single-base substitutions in vitro at high frequency.Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.Isolation and characterization of mutations in the HXK2 gene of Saccharomyces cerevisiae.Characterization of a surrogate TATA box promoter that regulates in vitro transcription of the simian virus 40 major late geneA single Saccharomyces cerevisiae upstream activation site (UAS1) has two distinct regions essential for its activityA fidelity assay using "dideoxy" DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.Two-codon insertion mutagenesis of plasmid genes by using single-stranded hexameric oligonucleotidesBeta-lactamases: molecular studies.Novel quinolone resistance mutations of the Escherichia coli DNA gyrase A protein: enzymatic analysis of the mutant proteins.Tailoring the activity of restriction endonuclease PleI by PNA-induced DNA looping.The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5' of an ATG initiation codon.Synthesis and separation of diastereomers of deoxynucleoside 5'-O-(1-thio)triphosphates.A general method to select for M13 clones carrying base pair substitution mutants constructed in vitroAn easy and fast alternative to plasmid shuffling for the identification of in vitro mutagenized alleles of essential genes of Saccharomyces cerevisiae.A mechanism for deletion formation in DNA by human cell extracts: the involvement of short sequence repeats.Strand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromideA simple and efficient method for chemical mutagenesis of DNA.
P2860
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P2860
Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.
description
1982 nî lūn-bûn
@nan
1982年の論文
@ja
1982年学术文章
@wuu
1982年学术文章
@zh-cn
1982年学术文章
@zh-hans
1982年学术文章
@zh-my
1982年学术文章
@zh-sg
1982年學術文章
@yue
1982年學術文章
@zh
1982年學術文章
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name
Gap misrepair mutagenesis: eff ...... frameshift mutations in vitro.
@ast
Gap misrepair mutagenesis: eff ...... frameshift mutations in vitro.
@en
type
label
Gap misrepair mutagenesis: eff ...... frameshift mutations in vitro.
@ast
Gap misrepair mutagenesis: eff ...... frameshift mutations in vitro.
@en
prefLabel
Gap misrepair mutagenesis: eff ...... frameshift mutations in vitro.
@ast
Gap misrepair mutagenesis: eff ...... frameshift mutations in vitro.
@en
P2093
P2860
P356
P1476
Gap misrepair mutagenesis: eff ...... frameshift mutations in vitro.
@en
P2093
S J Benkovic
P2860
P304
P356
10.1073/PNAS.79.5.1588
P407
P577
1982-03-01T00:00:00Z