Production of unmodified human adult hemoglobin in Escherichia coli
about
Development of recombinant hemoglobin-based oxygen carriersA novel two-over-two alpha-helical sandwich fold is characteristic of the truncated hemoglobin familyBlocking the Gate to Ligand Entry in Human HemoglobinA recombinant polymeric hemoglobin with conformational, functional, and physiological characteristics of an in vivo O2 transporterA biochemical--biophysical study of hemoglobins from woolly mammoth, Asian elephant, and humansDistal histidine stabilizes bound O2 and acts as a gate for ligand entry in both subunits of adult human hemoglobin.An investigation of the distal histidyl hydrogen bonds in oxyhemoglobin: effects of temperature, pH, and inositol hexaphosphate.Expression and purification of recombinant hemoglobin in Escherichia coli.Substitutions in woolly mammoth hemoglobin confer biochemical properties adaptive for cold tolerance.Chain-selective isotopic labeling for NMR studies of large multimeric proteins: application to hemoglobin.Cysteines beta93 and beta112 as probes of conformational and functional events at the human hemoglobin subunit interfaces.AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded stateNew look at hemoglobin allostery.Restoring allosterism with compensatory mutations in hemoglobinExpression and purification of active mouse and human NEIL3 proteins.Sickle Cell Hemoglobin with Mutation at αHis-50 Has Improved Solubility.Stable octameric structure of recombinant hemoglobin alpha(2)beta(2)83 Gly-->Cys.Strategies for achieving high-level expression of genes in Escherichia coli.Staphylococcus aureus growth using human hemoglobin as an iron sourceA comparative NMR study of the polypeptide backbone dynamics of hemoglobin in the deoxy and carbonmonoxy formsPost-translational transformation of methionine to aspartate is catalyzed by heme iron and driven by peroxide: a novel subunit-specific mechanism in hemoglobinInteraction of haptoglobin with hemoglobin octamers based on the mutation αAsn78Cys or βGly83Cys.Relationship between beta4 hydrogen bond and beta6 hydrophobic interactions during aggregate, fiber or crystal formation in oversaturated solutions of hemoglobin A and SAutoxidation and oxygen binding properties of recombinant hemoglobins with substitutions at the αVal-62 or βVal-67 position of the distal heme pocketInterfacial and distal-heme pocket mutations exhibit additive effects on the structure and function of hemoglobin.NMR reveals hydrogen bonds between oxygen and distal histidines in oxyhemoglobin.Role of β/δ101Gln in regulating the effect of temperature and allosteric effectors on oxygen affinity in woolly mammoth hemoglobin.Co-expression for intracellular processing in microbial protein production.Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli.Structure-function relationship in a variant hemoglobin: a combined computational-experimental approach.Co-expression of ferrochelatase allows for complete heme incorporation into recombinant proteins produced in E. coli.Haem disorder in recombinant- and reticulocyte-derived haemoglobins: evidence for stereoselective haem insertion in eukaryotes.Properties of a recombinant human hemoglobin double mutant: sickle hemoglobin with Leu-88(beta) at the primary aggregation site substituted by Ala.Co-expression of glutathione S-transferase with methionine aminopeptidase: a system of producing enriched N-terminal processed proteins in Escherichia coli.Hemoglobin Kirklareli (α H58L), a New Variant Associated with Iron Deficiency and Increased CO Binding.Role of beta112 Cys (G14) in homo- (beta4) and hetero- (alpha2 beta2) tetramer hemoglobin formation.Indefinite noncooperative self-association of chicken deoxy hemoglobin D.Efficacy of recombinant human Hb by 31P-NMR during isovolemic total exchange transfusion.Possibilities of Using Fetal Hemoglobin as a Platform for Producing Hemoglobin-Based Oxygen Carriers (HBOCs).Heme Orientation of Cavity Mutant Hemoglobins (His F8 → Gly) in Either α or β Subunits: Circular Dichroism, (1) H NMR, and Resonance Raman Studies.
P2860
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P2860
Production of unmodified human adult hemoglobin in Escherichia coli
description
1993 nî lūn-bûn
@nan
1993年の論文
@ja
1993年論文
@yue
1993年論文
@zh-hant
1993年論文
@zh-hk
1993年論文
@zh-mo
1993年論文
@zh-tw
1993年论文
@wuu
1993年论文
@zh
1993年论文
@zh-cn
name
Production of unmodified human adult hemoglobin in Escherichia coli
@ast
Production of unmodified human adult hemoglobin in Escherichia coli
@en
type
label
Production of unmodified human adult hemoglobin in Escherichia coli
@ast
Production of unmodified human adult hemoglobin in Escherichia coli
@en
prefLabel
Production of unmodified human adult hemoglobin in Escherichia coli
@ast
Production of unmodified human adult hemoglobin in Escherichia coli
@en
P2093
P2860
P356
P1476
Production of unmodified human adult hemoglobin in Escherichia coli
@en
P2093
P2860
P304
P356
10.1073/PNAS.90.17.8108
P407
P50
P577
1993-09-01T00:00:00Z