Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes. - Wikidata - awgldk - TriplyDB

Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes.

Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes.

http://www.wikidata.org/entity/Q36557775

about

Programmed evolution for optimization of orthogonal metabolic output in bacteria Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei High-copy bacterial plasmids diffuse in the nucleoid-free space, replicate stochastically and are randomly partitioned at cell division.Conjugative Type 4 secretion system of a novel large plasmid from the chemoautotroph Tetrathiobacter kashmirensis and construction of shuttle vectors for Alcaligenaceae.Determination of plasmid copy number reveals the total plasmid DNA amount is greater than the chromosomal DNA amount in Bacillus thuringiensis YBT-1520 BglBrick vectors and datasheets: A synthetic biology platform for gene expression.Dynamic modulation of DNA replication and gene transcription in deep-sea filamentous phage SW1 in response to changes of host growth and temperature The purine-utilizing bacterium Clostridium acidurici 9a: a genome-guided metabolic reconsideration Acquisition through horizontal gene transfer of plasmid pSMA198 by Streptococcus macedonicus ACA-DC 198 points towards the dairy origin of the species.Heterologous protein secretion in Lactobacilli with modified pSIP vectors.pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system Expression of Lactate Dehydrogenase in Aspergillus niger for L-Lactic Acid Production.Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum.Plasmid Copy Number Determination by Quantitative Polymerase Chain Reaction Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR Rampant Parasexuality Evolves in a Hospital Pathogen during Antibiotic Selection.Comparison of six commercial kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing.Microarray and functional analysis of growth phase-dependent gene regulation in Bordetella bronchiseptica.Generation of a stable plasmid for in vitro and in vivo studies of Staphylococcus.Evaluation of CTX-M steady-state mRNA, mRNA half-life and protein production in various STs of Escherichia coli Escherichia coli O157:H7 Strains Isolated from High-Event Period Beef Contamination Have Strong Biofilm-Forming Ability and Low Sanitizer Susceptibility, Which Are Associated with High pO157 Plasmid Copy Number.Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR Combined effect of improved cell yield and increased specific productivity enhances recombinant enzyme production in genome-reduced Bacillus subtilis strain MGB874.ZpdN, a Plasmid-Encoded Sigma Factor Homolog, Induces pBS32-Dependent Cell Death in Bacillus subtilis.Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum.Taking phage integration to the next level as a genetic tool for mycobacteria A model for the evolution of biological specificity: a cross-reacting DNA-binding protein causes plasmid incompatibility.bla(KPC) RNA expression correlates with two transcriptional start sites but not always with gene copy number in four genera of Gram-negative pathogens Quantitative Localization Microscopy Reveals a Novel Organization of a High-Copy Number Plasmid Characterization of plasmid pML21 of Enterococcus faecalis ML21 from koumiss.The seed composition of Arabidopsis mutants for the group 3 sulfate transporters indicates a role in sulfate translocation within developing seeds.The scientific impact of microbial cell factories.Synchronized DNA cycling across a bacterial population.Using the CRISPR/Cas9 system to eliminate native plasmids of Zymomonas mobilis ZM4.Development of a high-copy-number plasmid via adaptive laboratory evolution of Corynebacterium glutamicum.Multiplex recording of cellular events over time on CRISPR biological tape.A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3).Plasmid transfer efficiency using Lactoccocus lactis strains depends on invasiveness status but also on plasmid copy number.A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction.

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P2860

Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes.

http://www.wikidata.org/entity/Q36557775

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2008 nî lūn-bûn

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Improved determination of plas ...... toring fermentation processes.

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Improved determination of plas ...... toring fermentation processes.

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Microbial Cell Factories

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Improved determination of plas ...... toring fermentation processes.

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P2093

Borut Strukelj

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Mihaela Skulj

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Petra Slanc

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Simona Jevsevar

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Spela Jalen

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Veronika Okrslar

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Viktor Menart

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P2860

Estimating the number of integrations in transformed plants by quantitative real-time PCR.

Real-time PCR in clinical microbiology: applications for routine laboratory testing.

A new mathematical model for relative quantification in real-time RT-PCR

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

PCR technology for screening and quantification of genetically modified organisms (GMOs)

Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.

Impact of targeted vector design on Co/E1 plasmid replication.

Plasmid copy number and plasmid stability.

Detection and traceability of genetically modified organisms in the food production chain.

The replication-related organization of bacterial genomes.

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10.1186/1475-2859-7-6

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