Engineered turns of a recombinant antibody improve its in vivo folding.
about
Procaryotic expression of single-chain variable-fragment (scFv) antibodies: secretion in L-form cells of Proteus mirabilis leads to active product and overcomes the limitations of periplasmic expression in Escherichia coliIn vitro selection and evolution of functional proteins by using ribosome displayOligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display librarySelection, affinity maturation, and characterization of a human scFv antibody against CEA proteinStrategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.Destabilizing loop swaps in the CDRs of an immunoglobulin VL domain.The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines.IVIG-bound IgG and IgM cloned by phage display from a healthy individual reveal the same restricted germ-line gene origin as in autoimmune thrombocytopenia.Genetic origin of IgG antibodies cloned by phage display and anti-idiotypic panning from three patients with autoimmune thrombocytopenia.Design of synthetic antibody libraries.Intracellular immunization of prokaryotic cells against a bacteriotoxin.Expression studies of catalytic antibodies.Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays.Expression of a Functional zipFv Antibody Fragment and Its Fusions with Alkaline Phosphatase in the Cytoplasm of an Escherichia coli.Symmetry of Fv architecture is conducive to grafting a second antibody binding site in the Fv region.Contributions of a highly conserved VH/VL hydrogen bonding interaction to scFv folding stability and refolding efficiency.Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies.Bacterial Inner-membrane Display for Screening a Library of Antibody FragmentsN-terminal mutations in the anti-estradiol Fab 57-2 modify its hapten binding propertiesStrategies for achieving high-level expression of genes in Escherichia coli.Antibodies to steroids from a small human naive IgM library.Antiferritin single-chain antibody: a functional protein with incomplete folding?High efficient expression of a functional humanized single-chain variable fragment (scFv) antibody against CD22 in Pichia pastoris.Enhanced secretory production of a single-chain antibody fragment from Bacillus subtilis by coproduction of molecular chaperones.A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris.PEGylation and multimerization of the anti-p185HER-2 single chain Fv fragment 4D5: effects on tumor targeting.Mutations can cause light chains to be too stable or too unstable to form amyloid fibrils.Bacterial surface display of an anti-pollutant antibody fragment.Studies of single-chain antibody expression in quiescent Escherichia coli.Catalytic antibodies--reaching adolescence?Rescue of a neutralizing anti-viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells.Elongation of the C-terminal domain of an anti-amyloid β single-chain variable fragment increases its thermodynamic stability and decreases its aggregation tendency.Direct evidence by H/D exchange and ESI-MS for transient unproductive domain interaction in the refolding of an antibody scFv fragment.Kinetic model of in vivo folding and inclusion body formation in recombinant Escherichia coli.Mutational analysis of domain antibodies reveals aggregation hotspots within and near the complementarity determining regions.Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris.Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells.Anti-digoxin scFv fragments expressed in bacteria and in insect cells have different antigen binding properties.Co-selection of cognate antibody-antigen pairs by selectively-infective phages.
P2860
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P2860
Engineered turns of a recombinant antibody improve its in vivo folding.
description
1995 nî lūn-bûn
@nan
1995年の論文
@ja
1995年論文
@yue
1995年論文
@zh-hant
1995年論文
@zh-hk
1995年論文
@zh-mo
1995年論文
@zh-tw
1995年论文
@wuu
1995年论文
@zh
1995年论文
@zh-cn
name
Engineered turns of a recombinant antibody improve its in vivo folding.
@ast
Engineered turns of a recombinant antibody improve its in vivo folding.
@en
type
label
Engineered turns of a recombinant antibody improve its in vivo folding.
@ast
Engineered turns of a recombinant antibody improve its in vivo folding.
@en
prefLabel
Engineered turns of a recombinant antibody improve its in vivo folding.
@ast
Engineered turns of a recombinant antibody improve its in vivo folding.
@en
P356
P1476
Engineered turns of a recombinant antibody improve its in vivo folding.
@en
P2093
Plückthun A
P356
10.1093/PROTEIN/8.1.81
P577
1995-01-01T00:00:00Z