Positive and negative roles of an initiator protein at an origin of replication.
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Origin pairing ('handcuffing') as a mode of negative control of P1 plasmid copy numberProduction of combinatorial libraries of fused genes by sequential transposition reactionsSingle-stranded heteroduplex intermediates in lambda Red homologous recombination.Two forms of replication initiator protein: positive and negative controls.Role of pi dimers in coupling ("handcuffing") of plasmid R6K's gamma ori iterons.Negative control of plasmid R6K replication: possible role of intermolecular coupling of replication origins.Initiator protein pi can bind independently to two domains of the gamma origin core of plasmid R6K: the direct repeats and the A+T-rich segmentPaperClip: rapid multi-part DNA assembly from existing libraries.Multiple pathways of copy control of gamma replicon of R6K: mechanisms both dependent on and independent of cooperativity of interaction of tau protein with DNA affect the copy number.A DNA segment conferring stable maintenance on R6K gamma-origin core replicons.Preponderance of Fis-binding sites in the R6K gamma origin and the curious effect of the penicillin resistance marker on replication of this origin in the absence of FisInteractions of the RepA1 protein with its replicon targets: two opposing roles in control of plasmid replication.Roles of long and short replication initiation proteins in the fate of IncP-1 plasmids.Structure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interfaceBinding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi proteinThe integration host factor of Escherichia coli binds to multiple sites at plasmid R6K gamma origin and is essential for replication.Functional characterization of a replication initiator proteinIdentification of a novel promoter in the replication control region of plasmid R6KEffects of mutations in the repA gene of plasmid Rts1 on plasmid replication and autorepressor function.P1 plasmid replication: initiator sequestration is inadequate to explain control by initiator-binding sites.P1 plasmid replication: measurement of initiator protein concentration in vivo.Location of a P1 plasmid replication inhibitor determinant within the initiator geneReplication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy numberpi protein- and ATP-dependent transitions from 'closed' to 'open' complexes at the gamma ori of plasmid R6KCooperative binding mode of the inhibitors of R6K replication, pi dimers.pNEB193-derived suicide plasmids for gene deletion and protein expression in the methane-producing archaeon, Methanosarcina acetivoransPlasmid R6K replication controlReplication and conjugative mobilization of broad host-range IncQ plasmids.Gene replacement techniques for Escherichia coli genome modification.Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning.Requirements for and regulation of origin opening of plasmid P1.Site-directed mutations in the repA C-terminal region of plasmid Rts1: pleiotropic effects on the replication and autorepressor functions.Replication of plasmids in gram-negative bacteria.Multiple origins and replication proteins influence biological properties of beta-lactamase-producing plasmids from Neisseria gonorrhoeae.Replication and control of circular bacterial plasmids.Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.Integration host factor of Escherichia coli reverses the inhibition of R6K plasmid replication by pi initiator protein.Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression.
P2860
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P2860
Positive and negative roles of an initiator protein at an origin of replication.
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on December 1986
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
Positive and negative roles of an initiator protein at an origin of replication.
@en
Positive and negative roles of an initiator protein at an origin of replication.
@nl
type
label
Positive and negative roles of an initiator protein at an origin of replication.
@en
Positive and negative roles of an initiator protein at an origin of replication.
@nl
prefLabel
Positive and negative roles of an initiator protein at an origin of replication.
@en
Positive and negative roles of an initiator protein at an origin of replication.
@nl
P2093
P2860
P356
P1476
Positive and negative roles of an initiator protein at an origin of replication.
@en
P2093
D R Helinski
M Filutowicz
M J McEachern
P2860
P304
P356
10.1073/PNAS.83.24.9645
P407
P577
1986-12-01T00:00:00Z