Prokaryotic expression of the heme- and flavin-binding domains of rat neuronal nitric oxide synthase as distinct polypeptides: identification of the heme-binding proximal thiolate ligand as cysteine-415.
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Nitric oxide synthases: structure, function and inhibitionKinetics of CO binding to the haem domain of murine inducible nitric oxide synthase: differential effects of haem domain ligandsSubunit interactions of endothelial nitric-oxide synthase. Comparisons to the neuronal and inducible nitric-oxide synthase isoformsCarbon monoxide: from toxin to endogenous modulator of cardiovascular functions.Inducible nitric oxide synthase: identification of amino acid residues essential for dimerization and binding of tetrahydrobiopterinThe autoinhibitory control element and calmodulin conspire to provide physiological modulation of endothelial and neuronal nitric oxide synthase activity.High-level expression of functional rat neuronal nitric oxide synthase in Escherichia coli.Mutation of Glu-361 in human endothelial nitric-oxide synthase selectively abolishes L-arginine binding without perturbing the behavior of heme and other redox centersCysteine-mediated redox signaling: chemistry, biology, and tools for discoveryEnterohemorrhagic Escherichia coli O157: epidemiology and ecology in bovine production environments.Expression and immunoaffinity purification of human inducible nitric-oxide synthase. Inhibition studies with 2-amino-5,6-dihydro-4H-1,3-thiazine.Delineation of the arginine- and tetrahydrobiopterin-binding sites of neuronal nitric oxide synthase.Nitric oxide synthase domain interfaces regulate electron transfer and calmodulin activation.A professional and personal odyssey.Neuronal nitric oxide synthase and dystrophin-deficient muscular dystrophy.DINITROBENZENES STIMULATE ELECTRON FLUX WITHIN NEURONAL NITRIC OXIDE SYNTHASE IN THE ABSENCE OF CALMODULIN.Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes.Phosphorylation within an autoinhibitory domain in endothelial nitric oxide synthase reduces the Ca(2+) concentrations required for calmodulin to bind and activate the enzyme.Removal of a putative inhibitory element reduces the calcium-dependent calmodulin activation of neuronal nitric-oxide synthase.Nitric-oxide synthase (NOS) reductase domain models suggest a new control element in endothelial NOS that attenuates calmodulin-dependent activity.Characterization of the reductase domain of rat neuronal nitric oxide synthase generated in the methylotrophic yeast Pichia pastoris. Calmodulin response is complete within the reductase domain itself.Redox properties of human endothelial nitric-oxide synthase oxygenase and reductase domains purified from yeast expression system.Characterization of the roles of the 594-645 region in human endothelial nitric-oxide synthase in regulating calmodulin binding and electron transfer.L-arginine binding to nitric-oxide synthase. The role of H-bonds to the nonreactive guanidinium nitrogens.Heme iron reduction and catalysis by a nitric oxide synthase heterodimer containing one reductase and two oxygenase domains.Reduction of quinonoid dihydrobiopterin to tetrahydrobiopterin by nitric oxide synthase.Characterization by electron paramagnetic resonance of the interactions of L-arginine and L-thiocitrulline with the heme cofactor region of nitric oxide synthase.Oligomerization of endothelial nitric oxide synthase. Evidence for a dominant negative effect of truncation mutants.Kinetics of NO ligation with nitric-oxide synthase by flash photolysis and stopped-flow spectrophotometry.Nitroarginine and tetrahydrobiopterin binding to the haem domain of neuronal nitric oxide synthase using a scintillation proximity assay.Effects of combined phosphorylation at Ser-617 and Ser-1179 in endothelial nitric-oxide synthase on EC50(Ca2+) values for calmodulin binding and enzyme activationImproved method for assembly of hemeprotein neuronal NO-synthase heterodimers.Activation of neuronal nitric-oxide synthase by the 5-methyl analog of tetrahydrobiopterin. Functional evidence against reductive oxygen activation by the pterin cofactor.Overexpression of neuronal nitric oxide synthase in insect cells reveals requirement of haem for tetrahydrobiopterin binding.Cysteine-200 of human inducible nitric oxide synthase is essential for dimerization of haem domains and for binding of haem, nitroarginine and tetrahydrobiopterin.Allosteric modulation of rat brain nitric oxide synthase by the pterin-site enzyme inhibitor 4-aminotetrahydrobiopterin.Stopped-flow kinetic studies of electron transfer in the reductase domain of neuronal nitric oxide synthase: re-evaluation of the kinetic mechanism reveals new enzyme intermediates and variation with cytochrome P450 reductase.Structure diversity of nitric oxide synthases (NOS): the emergence of new forms in photosynthetic organisms.Control of electron transfer in nitric-oxide synthases. Swapping of autoinhibitory elements among nitric-oxide synthase isoforms.Rapid calmodulin-dependent interdomain electron transfer in neuronal nitric-oxide synthase measured by pulse radiolysis.
P2860
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P2860
Prokaryotic expression of the heme- and flavin-binding domains of rat neuronal nitric oxide synthase as distinct polypeptides: identification of the heme-binding proximal thiolate ligand as cysteine-415.
description
1995 nî lūn-bûn
@nan
1995年の論文
@ja
1995年論文
@yue
1995年論文
@zh-hant
1995年論文
@zh-hk
1995年論文
@zh-mo
1995年論文
@zh-tw
1995年论文
@wuu
1995年论文
@zh
1995年论文
@zh-cn
name
Prokaryotic expression of the ...... iolate ligand as cysteine-415.
@en
type
label
Prokaryotic expression of the ...... iolate ligand as cysteine-415.
@en
prefLabel
Prokaryotic expression of the ...... iolate ligand as cysteine-415.
@en
P356
P1433
P1476
Prokaryotic expression of the ...... iolate ligand as cysteine-415.
@en
P2093
Masters BS
McMillan K
P304
P356
10.1021/BI00011A025
P407
P577
1995-03-01T00:00:00Z