Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases.
about
The Generation and Exploitation of Protein Mutability Landscapes for Enzyme EngineeringThermal stability and kinetic constants for 129 variants of a family 1 glycoside hydrolase reveal that enzyme activity and stability can be separately designedSingle-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encodedMutations Closer to the Active Site Improve the Promiscuous Aldolase Activity of 4-Oxalocrotonate Tautomerase More Effectively than Distant Mutations.Engineering a Promiscuous Tautomerase into a More Efficient Aldolase for Self-Condensations of Linear Aliphatic Aldehydes.Deep sequencing methods for protein engineering and design.Stereochemical Control of Enzymatic Carbon-Carbon Bond-Forming Michael-Type Additions by "Substrate Engineering".A global view of structure-function relationships in the tautomerase superfamily.Gram-Scale Synthesis of Chiral Cyclopropane-Containing Drugs and Drug Precursors with Engineered Myoglobin Catalysts Featuring Complementary Stereoselectivity.Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy.Catalysis of amorpha-4,11-diene synthase unraveled and improved by mutability landscape guided engineering.
P2860
Q28070023-D9CC8AC4-154E-475E-89B1-EA852414348EQ30151137-0740D715-58F0-4E4C-87DC-EE2AA1C1447DQ33786307-F4233940-23A1-49C5-B926-E1130FFDDF68Q36033515-89859E48-799F-4F5F-846E-1B4872D8AF00Q36351769-04D3FA33-1AC3-4FFB-9413-A4F0423F4572Q39019516-178B7C87-B973-4AE0-BEF3-9AE6292F36B1Q42366816-04F4DA5E-535B-41DD-816D-BF7D80E71F59Q46026170-FA2DD5C7-9613-4B85-A33C-1C7087A1F817Q47099176-0266F7A7-093D-4E18-9901-42162C3292AEQ55424759-7D6A789D-EBEC-466F-BE4D-75B5C95C2D80Q55517590-5676C604-DAED-4604-9BDC-744383246490
P2860
Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases.
description
2016 nî lūn-bûn
@nan
2016年の論文
@ja
2016年学术文章
@wuu
2016年学术文章
@zh-cn
2016年学术文章
@zh-hans
2016年学术文章
@zh-my
2016年学术文章
@zh-sg
2016年學術文章
@yue
2016年學術文章
@zh
2016年學術文章
@zh-hant
name
Using mutability landscapes of ...... enantioselective Michaelases.
@en
type
label
Using mutability landscapes of ...... enantioselective Michaelases.
@en
prefLabel
Using mutability landscapes of ...... enantioselective Michaelases.
@en
P2093
P2860
P356
P1476
Using mutability landscapes of ...... enantioselective Michaelases.
@en
P2093
Andreas Kunzendorf
Bert-Jan Baas
Edzard M Geertsema
Harshwardhan Poddar
Jan-Ytzen van der Meer
Mehran Rahimi
Pieter G Tepper
Ronald van Merkerk
Wim J Quax
Yufeng Miao
P2860
P2888
P356
10.1038/NCOMMS10911
P407
P577
2016-03-08T00:00:00Z
P5875
P6179
1039800485