PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. - Wikidata - awgldk - TriplyDB

PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.

PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.

http://www.wikidata.org/entity/Q39528384

about

A genome-wide analysis of small regulatory RNAs in the human pathogen group A Streptococcus A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast Advances in Aspergillus secondary metabolite research in the post-genomic era Crystal Structure of Peroxide Stress Regulator from Streptococcus pyogenes Provides Functional Insights into the Mechanism of Oxidative Stress Sensing An organic acid-tolerant HAA1-overexpression mutant of an industrial bioethanol strain of Saccharomyces cerevisiae and its application to the production of bioethanol from sugarcane molasses A PLAC8-containing protein from an endomycorrhizal fungus confers cadmium resistance to yeast cells by interacting with Mlh3p A KaiC-associating SasA-RpaA two-component regulatory system as a major circadian timing mediator in cyanobacteria Comparative genomics to explore phylogenetic relationship, cryptic sexual potential and host specificity of Rhynchosporium species on grasses The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.The Structure of a Type 3 Secretion System (T3SS) Ruler Protein Suggests a Molecular Mechanism for Needle Length Sensing Role of OmpA2 surface regions of Porphyromonas gingivalis in host-pathogen interactions with oral epithelial cells.gamma-tubulin plays an essential role in the coordination of mitotic events.Optimization of a large-scale gene disruption protocol in Dictyostelium and analysis of conserved genes of unknown function.Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis.Cas3p belongs to a seven-member family of capsule structure designer proteins.UGD1, encoding the Cryptococcus neoformans UDP-glucose dehydrogenase, is essential for growth at 37 degrees C and for capsule biosynthesis Fusobacterium nucleatum-associated beta-defensin inducer (FAD-I): identification, isolation, and functional evaluation Vegetative incompatibility loci with dedicated roles in allorecognition restrict mycovirus transmission in chestnut blight fungus.Two functionally distinctive phosphopantetheinyl transferases from amoeba Dictyostelium discoideum PFP1, a gene encoding an Epc-N domain-containing protein, is essential for pathogenicity of the barley pathogen Rhynchosporium commune.Involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of Gordonia polyisoprenivorans strain VH2 Sorbitol required for cell growth and ethanol production by Zymomonas mobilis under heat, ethanol, and osmotic stresses.The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus.CovS inactivates CovR and is required for growth under conditions of general stress in Streptococcus pyogenes Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging A versatile and efficient gene-targeting system for Aspergillus nidulans.A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.Naturally occurring single amino acid replacements in a regulatory protein alter streptococcal gene expression and virulence in mice.Maltodextrin utilization plays a key role in the ability of group A Streptococcus to colonize the oropharynx A RabGAP regulates life-cycle duration via trimeric G-protein cascades in Dictyostelium discoideum.Genetic exchange of fimbrial alleles exemplifies the adaptive virulence strategy of Porphyromonas gingivalis.Niche-specific contribution to streptococcal virulence of a MalR-regulated carbohydrate binding protein.Molecular mechanisms of hypoxic responses via unique roles of Ras1, Cdc24 and Ptp3 in a human fungal pathogen Cryptococcus neoformans Porphyromonas gingivalis genes involved in community development with Streptococcus gordonii.Molecular characterization of vegetative incompatibility genes that restrict hypovirus transmission in the chestnut blight fungus Cryphonectria parasitica Differences between Cryptococcus neoformans and Cryptococcus gattii in the Molecular Mechanisms Governing Utilization of D-Amino Acids as the Sole Nitrogen Source.Natural competence is a major mechanism for horizontal DNA transfer in the oral pathogen Porphyromonas gingivalis DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites Involvement of PDK1, PKC and TOR signalling pathways in basal fluconazole tolerance in Cryptococcus neoformans.Murine model of dextran sulfate sodium-induced colitis reveals Candida glabrata virulence and contribution of β-mannosyltransferases.

P2860

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P2860

PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.

http://www.wikidata.org/entity/Q39528384

description

2002 nî lūn-bûn

@nan

2002年の論文

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2002年論文

@yue

2002年論文

@zh-hant

2002年論文

@zh-hk

2002年論文

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2002年論文

@zh-tw

2002年论文

@wuu

2002年论文

@zh

2002年论文

@zh-cn

name

PCR-mediated generation of a g ...... NA ligase and plasmid vectors.

@en

PCR-mediated generation of a g ...... NA ligase and plasmid vectors.

@nl

type

label

PCR-mediated generation of a g ...... NA ligase and plasmid vectors.

@en

PCR-mediated generation of a g ...... NA ligase and plasmid vectors.

@nl

prefLabel

PCR-mediated generation of a g ...... NA ligase and plasmid vectors.

@en

PCR-mediated generation of a g ...... NA ligase and plasmid vectors.

@nl

P1433

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P1433

Nucleic Acids Research

P1476

PCR-mediated generation of a g ...... NA ligase and plasmid vectors.

@en

P2093

Hidekazu Kuwayama

http://www.w3.org/2001/XMLSchema#string

Hideko Urushihara

http://www.w3.org/2001/XMLSchema#string

Mariko Katoh

http://www.w3.org/2001/XMLSchema#string

Shinji Obara

http://www.w3.org/2001/XMLSchema#string

Takahiro Morio

http://www.w3.org/2001/XMLSchema#string

Yoshimasa Tanaka

http://www.w3.org/2001/XMLSchema#string

P2860

A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae

The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development.

PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae.

Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.

Glycogen synthase kinase 3 regulates cell fate in Dictyostelium.

A developmentally regulated, putative serine/threonine protein kinase is essential for development in Dictyostelium.

Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction.

A transformation vector for dictyostelium discoideum with a new selectable marker bsr.

Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination.

P304

E2

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scholarly article

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10.1093/NAR/30.2.E2

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P433

2

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P478

30

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279333598

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11788728

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99841

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