about
Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix associationInteins and affinity resin substitutes for protein purification and scale upCrystal structures of the free and inhibited forms of plasmepsin I (PMI) from Plasmodium falciparumTobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiencyHigh-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1Maltose-binding protein fusion allows for high level bacterial expression and purification of bioactive mammalian cytokine derivativesModule structure of interphotoreceptor retinoid-binding protein (IRBP) may provide bases for its complex role in the visual cycle - structure/function study of Xenopus IRBP.Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins.Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins.Expression of active recombinant human tissue-type plasminogen activator by using in vivo polyhydroxybutyrate granule display.Production and characterization of a recombinant single-chain antibody against Hantaan virus envelop glycoprotein.Production of active mammalian and viral proteases in bacterial expression systems.Mechanism of action of recombinant acc-royalisin from royal jelly of Asian honeybee against gram-positive bacteria.Tenascin C promiscuously binds growth factors via its fifth fibronectin type III-like domain.Production of Nα-acetylated thymosin α1 in Escherichia coli.Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system.Enhanced Mitogenic Activity of Recombinant Human Vascular Endothelial Growth Factor VEGF121 Expressed in E. coli Origami B (DE3) with Molecular Chaperones.Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application.Plasmodium falciparum ookinete expression of plasmepsin VII and plasmepsin X.Strategies for achieving high-level expression of genes in Escherichia coli.Therapeutic and analytical applications of arsenic binding to proteins.Designing a recombinant Bacmid construct of HCV core+1 in Baculovirus expression system.Prokaryotic expression of a novel mouse pro-apoptosis protein PNAS-4 and application of its polyclonal antibodies.Hirudin as a novel fusion tag for efficient production of lunasin in Escherichia coli.In vivo immobilization of fusion proteins on bioplastics by the novel tag BioF.Sequence analysis and bacterial production of the anti-c-myc antibody 9E10: the V(H) domain has an extended CDR-H3 and exhibits unusual solubility.A novel method of affinity-purifying proteins using a bis-arsenical fluorescein.Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag.Expression of delta, kappa and mu human opioid receptors in Escherichia coli and reconstitution of the high-affinity state for agonist with heterotrimeric G proteins.Successful oral prime-immunization with VP60 from rabbit haemorrhagic disease virus produced in transgenic plants using different fusion strategies.Translocation of green fluorescent protein by comparative analysis with multiple signal peptides.Cystic fibrosis transmembrane conductance regulator: expression and helicity of a double membrane-spanning segment.Heterologous gene expression in bacterial systems under reduced oxygen tensions. Small-scale optimization precedes industrial fermentation.Expression and Activity Analysis of Fructosyltransferase from Aspergillus oryzae.Split Ssp DnaB mini-intein-mediated production of recombinant human glucagon-like peptide-1/7-36.Expression, purification, and characterization of mouse nesfatin-1 in Escherichia coli.Construction, expression, and characterization of Arabidopsis thaliana 4CL and Arachis hypogaea RS fusion gene 4CL::RS in Escherichia coli.Application of immobilized thrombin for production of S-thanatin expressed in Escherichia coli.Tobacco plastidial thioredoxins as modulators of recombinant protein production in transgenic chloroplasts
P2860
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P2860
description
1995 nî lūn-bûn
@nan
1995年の論文
@ja
1995年学术文章
@wuu
1995年学术文章
@zh-cn
1995年学术文章
@zh-hans
1995年学术文章
@zh-my
1995年学术文章
@zh-sg
1995年學術文章
@yue
1995年學術文章
@zh
1995年學術文章
@zh-hant
name
Gene fusion expression systems in Escherichia coli.
@en
type
label
Gene fusion expression systems in Escherichia coli.
@en
prefLabel
Gene fusion expression systems in Escherichia coli.
@en
P1476
Gene fusion expression systems in Escherichia coli.
@en
P2093
P304
P356
10.1016/0958-1669(95)80083-2
P577
1995-10-01T00:00:00Z