Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments
about
Rapid and efficient site-specific mutagenesis without phenotypic selectionRequirement for d(GATC) sequences in Escherichia coli mutHLS mismatch correctionProperties of a genetically engineered G domain of elongation factor Tu.An approach to construction of hybrid polypeptide molecules--homologue recombination methodChromogenic identification of oligonucleotide-directed mutantsOligonucleotide site-directed mutagenesis in Xenopus egg extracts.Methyl-directed repair of frameshift heteroduplexes in cell extracts from Escherichia coli.Heteroduplex deoxyribonucleic acid base mismatch repair in bacteria.Methyl-directed repair of DNA base-pair mismatches in vitroStructure-function relationships in the GTP binding domain of EF-Tu: mutation of Val20, the residue homologous to position 12 in p21Mutants of the membrane-binding region of Semliki Forest virus E2 protein. I. Cell surface transport and fusogenic activityHigh frequencies of short frameshifts in poly-CA/TG tandem repeats borne by bacteriophage M13 in Escherichia coli K-12.Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers.The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.The gapped duplex DNA approach to oligonucleotide-directed mutation construction.Introduction of restriction enzyme sites in protein-coding DNA sequences by site-specific mutagenesis not affecting the amino acid sequence: a computer programImproved oligonucleotide site-directed mutagenesis using M13 vectors.A general method to select for M13 clones carrying base pair substitution mutants constructed in vitroEfficient site-directed mutagenesis by simultaneous use of two primers.Solid phase in vitro mutagenesis using plasmid DNA template.Oligonucleotide-directed construction of mutations: a gapped duplex DNA procedure without enzymatic reactions in vitro.Mutations induced by DNA polymerase alpha upon in vitro replication of M13mp8(+) DNA.Yeast mutants temperature-sensitive for growth after random mutagenesis of the chromosomal RAS2 gene and deletion of the RAS1 gene.A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT.The molecular basis for rRNA-dependent spectinomycin resistance in Nicotiana chloroplasts.
P2860
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P2860
Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments
description
1982 nî lūn-bûn
@nan
1982年の論文
@ja
1982年学术文章
@wuu
1982年学术文章
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1982年学术文章
@zh-hans
1982年学术文章
@zh-my
1982年学术文章
@zh-sg
1982年學術文章
@yue
1982年學術文章
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1982年學術文章
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name
Directed mutagenesis of DNA cl ...... ery from restriction fragments
@en
type
label
Directed mutagenesis of DNA cl ...... ery from restriction fragments
@en
prefLabel
Directed mutagenesis of DNA cl ...... ery from restriction fragments
@en
P2093
P2860
P356
P1476
Directed mutagenesis of DNA cl ...... ery from restriction fragments
@en
P2093
P2860
P304
P356
10.1093/NAR/10.20.6475
P577
1982-10-01T00:00:00Z