Rapid and efficient site-specific mutagenesis without phenotypic selection
about
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.Crystal structure of the FMN-binding domain of human cytochrome P450 reductase at 1.93 A resolutionCrystal structure of a heparin- and integrin-binding segment of human fibronectin.Specific activation of leukocyte beta2 integrins lymphocyte function-associated antigen-1 and Mac-1 by chemokines mediated by distinct pathways via the alpha subunit cytoplasmic domainsPSK, a novel STE20-like kinase derived from prostatic carcinoma that activates the c-Jun N-terminal kinase mitogen-activated protein kinase pathway and regulates actin cytoskeletal organizationA novel aspartyl proteinase from apocrine epithelia and breast tumorsGeneration of novel cytoplasmic forms of protein tyrosine phosphatase epsilon by proteolytic processing and translational controlIdentification of sequences in the human peptide transporter subunit TAP1 required for transporter associated with antigen processing (TAP) functionExpression cloning of a novel zinc finger protein that binds to the c-fos serum response elementIdentification and characterization of GCP16, a novel acylated Golgi protein that interacts with GCP170The region of the HPV E7 oncoprotein homologous to adenovirus E1a and Sv40 large T antigen contains separate domains for Rb binding and casein kinase II phosphorylationDistinct mutations in two patients with leukocyte adhesion deficiency and their functional correlatesPhosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domainNon-SH2 domains within insulin receptor substrate-1 and SHC mediate their phosphotyrosine-dependent interaction with the NPEY motif of the insulin-like growth factor I receptorPhosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lckThe tumour suppressor gene product APC blocks cell cycle progression from G0/G1 to S phaseDistinct modes of interaction of SHC and insulin receptor substrate-1 with the insulin receptor NPEY region via non-SH2 domainsBlocking the Ca2+-induced conformational transitions in calmodulin with disulfide bondsOrganization and differential expression of the human monocyte chemoattractant protein 1 receptor gene. Evidence for the role of the carboxyl-terminal tail in receptor traffickingFunctional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologueCloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding proteinMammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coliD-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domainCloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesisIdentification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32Differential functions of the two Src homology 2 domains in protein tyrosine phosphatase SH-PTP1Analysis of the substrate binding sites of human galactosyltransferase by protein engineeringDevelopment of "substrate-trapping" mutants to identify physiological substrates of protein tyrosine phosphatasesCharacterization of an interaction between insulin receptor substrate 1 and the insulin receptor by using the two-hybrid systemPhosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56lck and its regulation by phosphorylation of Ser-59 in the lck unique N-terminal regionIdentification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirusOP-1 cDNA encodes an osteogenic protein in the TGF-beta familyCharacterization of the frameshift signal of Edr, a mammalian example of programmed -1 ribosomal frameshiftingEpidermal growth factor receptor and the adaptor protein p52Shc are specific substrates of T-cell protein tyrosine phosphatase.The MYND motif is required for repression of basal transcription from the multidrug resistance 1 promoter by the t(8;21) fusion proteinFunctional analysis of the CAAT box in the major late promoter of the subgroup C human adenovirusesIdentification of the predominant glycosaminoglycan-attachment site in soluble recombinant human thrombomodulin: potential regulation of functionality by glycosyltransferase competition for serine474Elimination of glycosylation heterogeneity affecting heparin affinity of recombinant human antithrombin III by expression of a beta-like variant in baculovirus-infected insect cellsOverexpression and site-directed mutagenesis of the succinyl-CoA synthetase of Escherichia coli and nucleotide sequence of a gene (g30) that is adjacent to the suc operonActivation of the Lbc Rho exchange factor proto-oncogene by truncation of an extended C terminus that regulates transformation and targeting
P2860
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P2860
Rapid and efficient site-specific mutagenesis without phenotypic selection
description
1985 nî lūn-bûn
@nan
1985 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
1985 թվականի հունվարին հրատարակված գիտական հոդված
@hy
1985年の論文
@ja
1985年論文
@yue
1985年論文
@zh-hant
1985年論文
@zh-hk
1985年論文
@zh-mo
1985年論文
@zh-tw
1985年论文
@wuu
name
Rapid and efficient site-specific mutagenesis without phenotypic selection
@ast
Rapid and efficient site-specific mutagenesis without phenotypic selection
@en
Rapid and efficient site-specific mutagenesis without phenotypic selection
@nl
type
label
Rapid and efficient site-specific mutagenesis without phenotypic selection
@ast
Rapid and efficient site-specific mutagenesis without phenotypic selection
@en
Rapid and efficient site-specific mutagenesis without phenotypic selection
@nl
prefLabel
Rapid and efficient site-specific mutagenesis without phenotypic selection
@ast
Rapid and efficient site-specific mutagenesis without phenotypic selection
@en
Rapid and efficient site-specific mutagenesis without phenotypic selection
@nl
P2860
P3181
P356
P1476
Rapid and efficient site-specific mutagenesis without phenotypic selection
@en
P2093
T A Kunkel
P2860
P304
P3181
P356
10.1073/PNAS.82.2.488
P407
P577
1985-01-01T00:00:00Z