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The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeastAn amino acid substitution in the pyruvate dehydrogenase E1 alpha gene, affecting mitochondrial import of the precursor proteinCrystal Structure of ChrR—A Quinone Reductase with the Capacity to Reduce ChromateThe design of a hyperstable mutant of the Abp1p SH3 domain by sequence alignment analysisEnzyme improvement in the absence of structural knowledge: a novel statistical approach.Genes involved in control of galactose uptake in Lactobacillus brevis and reconstitution of the regulatory system in Bacillus subtilis.Cation permeability and cation-anion interactions in a mutant GABA-gated chloride channel from Drosophila.Mammalian tropomodulins nucleate actin polymerization via their actin monomer binding and filament pointed end-capping activities.Diversity of cell envelope proteinase specificity among strains of Lactococcus lactis and its relationship to charge characteristics of the substrate-binding region.Addition of a class IIS enzyme site in the mutagenic primer to improve two-step PCR-based targeted mutagenesis.Analysis of novel soluble chromate and uranyl reductases and generation of an improved enzyme by directed evolutionDisease-causing mutations in exon 11 of the medium-chain acyl-CoA dehydrogenase geneDistribution and evolution of nisin-sucrose elements in Lactococcus lactisNegatively charged amino acid residues in the nicotinic receptor delta subunit that contribute to the binding of acetylcholine.Upstream stimulatory factors 1 and 2 mediate the transcription of angiotensin II binding and inhibitory protein.Stimulation of DNA inversion by FIS: evidence for enhancer-independent contacts with the Gin-gix complex.A cis-acting DNA element located between TATA box and transcription initiation site is critical in response to regulatory sequences in human angiotensinogen gene.Cold shock proteins and low-temperature response of Streptococcus thermophilus CNRZ302.Cloning, characterization, controlled overexpression, and inactivation of the major tributyrin esterase gene of Lactococcus lactis.Cold shock proteins of Lactococcus lactis MG1363 are involved in cryoprotection and in the production of cold-induced proteins.Molecular characterization of the Lactococcus lactis ptsHI operon and analysis of the regulatory role of HPrInvolvement of Runx3 in the basal transcriptional activation of the mouse angiotensin II type 1 receptor-associated protein gene.Optimization of bacteriocin release protein (BRP)-mediated protein release by Escherichia coli: random mutagenesis of the pCloDF13-derived BRP gene to uncouple lethality and quasi-lysis from protein release.Physiological activity of Campylobacter jejuni far below the minimal growth temperature.A tandem array of minimal U1 small nuclear RNA genes is sufficient to generate a new adenovirus type 12-inducible chromosome fragile site.An efficient PCR mutagenesis strategy without gel purification [correction of "purificiation"] step that is amenable to automation.Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering.Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin.Identical transcriptional control of the divergently transcribed prtP and prtM genes that are required for proteinase production in lactococcus lactis SK11.PU.1 (Spi-1) and C/EBP alpha regulate expression of the granulocyte-macrophage colony-stimulating factor receptor alpha gene.Intronic and flanking sequences are required to silence enhancement of an embryonic beta-type globin gene.YY1 and NF1 both activate the human p53 promoter by alternatively binding to a composite element, and YY1 and E1A cooperate to amplify p53 promoter activityPhysiological and regulatory effects of controlled overproduction of five cold shock proteins of Lactococcus lactis MG1363.Improved method for PCR-mediated site-directed mutagenesis.Molecular mechanism of transcriptional activation of angiotensinogen gene by proximal promoter.A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase.Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.A general and fast method to generate multiple site directed mutationsImproved primer design for PCR-based, site-directed mutagenesis.Functional expression of insecticide-resistant GABA receptors from the mosquito Aedes aegypti.
P2860
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P2860
description
1991 nî lūn-bûn
@nan
1991年の論文
@ja
1991年学术文章
@wuu
1991年学术文章
@zh-cn
1991年学术文章
@zh-hans
1991年学术文章
@zh-my
1991年学术文章
@zh-sg
1991年學術文章
@yue
1991年學術文章
@zh
1991年學術文章
@zh-hant
name
Improved site-directed mutagenesis method using PCR.
@en
type
label
Improved site-directed mutagenesis method using PCR.
@en
prefLabel
Improved site-directed mutagenesis method using PCR.
@en
P2093
P2860
P356
P1476
Improved site-directed mutagenesis method using PCR.
@en
P2093
P2860
P356
10.1093/NAR/19.16.4558
P577
1991-08-01T00:00:00Z