Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.
about
Structural and functional characterization of a noncanonical nucleoside triphosphate pyrophosphatase fromThermotoga maritimaStudies on the role of the hydrophobic domain of Ost4p in interactions with other subunits of yeast oligosaccharyl transferase.The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer.Reversible transdominant inhibition of a metabolic pathway. In vivo evidence of interaction between two sequential tricarboxylic acid cycle enzymes in yeast.Estimating the evidence of selection and the reliability of inference in unigenic evolution.Structural analysis of Brucella abortus RicA substitutions that do not impair interaction with human Rab2 GTPase.Dynein supports motility of endoplasmic reticulum in the fungus Ustilago maydisIdentification of residues in the N-terminal domain of the Yersinia tyrosine phosphatase that are critical for substrate recognition.Complementation analysis of the cold-sensitive phenotype of the Escherichia coli csdA deletion strain.RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase IIPhenotypic expression of PCR-generated random mutations in a Pseudomonas putida gene after its introduction into an Acinetobacter chromosome by natural transformationModeling the effects of prl mutations on the Escherichia coli SecY complex.A simple and reproducible method for directed evolution: combination of random mutation with dITP and DNA fragmentation with endonuclease V.Genetic analysis of the bacteriophage T4-encoded cochaperonin Gp31TIM23-mediated insertion of transmembrane α-helices into the mitochondrial inner membrane.Random mutagenesis of the arylacetonitrilase from Pseudomonas fluorescens EBC191 and identification of variants, which form increased amounts of mandeloamide from mandelonitrile.Identification and characterization of HsIV HsIU (ClpQ ClpY) proteins involved in overall proteolysis of misfolded proteins in Escherichia coli.Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V.Transcription antitermination by translation initiation factor IF1.Base-Modified Nucleic Acids as a Powerful Tool for Synthetic Biology and Biotechnology.Probing the structure and function of the estrogen receptor ligand binding domain by analysis of mutants with altered transactivation characteristics.Dislocation by the m-AAA protease increases the threshold hydrophobicity for retention of transmembrane helices in the inner membrane of yeast mitochondria.Ambiguous base pairing of the purine analogue 1-(2-deoxy-beta-D-ribofuranosyl)-imidazole-4-carboxamide during PCRPharmacogenetic significance of inosine triphosphatase.A conformational switch in the ligand-binding domain regulates the dependence of the glucocorticoid receptor on Hsp90.Interaction of Saccharomyces Cdc13p with Pol1p, Imp4p, Sir4p and Zds2p is involved in telomere replication, telomere maintenance and cell growth controlInosine triphosphate pyrophosphohydrolase (ITPA) polymorphic sequence variants in adult hematological malignancy patients and possible association with mitochondrial DNA defectsA new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machineryFunctional mapping of the EcoRV DNA methyltransferase by random mutagenesis and screening for catalytically inactive mutants.Stimulation of DNA inversion by FIS: evidence for enhancer-independent contacts with the Gin-gix complex.Analysis of the role of the EnvZ linker region in signal transduction using a chimeric Tar/EnvZ receptor protein, Tez1.Hypoxanthine incorporation is nonmutagenic in Escherichia coli.Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array.14-3-3 proteins are required for maintenance of Raf-1 phosphorylation and kinase activity.Functional mapping of the DNA binding domain of bovine papillomavirus E1 proteinSelective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymeraseChanging the recognition specificity of a DNA-methyltransferase by in vitro evolution.Combining localized PCR mutagenesis and natural transformation in direct genetic analysis of a transcriptional regulator gene, pobR.Suppression of mutant phenotypes of the Agrobacterium tumefaciens VirB11 ATPase by overproduction of VirB proteinsInteraction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain.
P2860
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P2860
Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.
description
1993 nî lūn-bûn
@nan
1993年の論文
@ja
1993年論文
@yue
1993年論文
@zh-hant
1993年論文
@zh-hk
1993年論文
@zh-mo
1993年論文
@zh-tw
1993年论文
@wuu
1993年论文
@zh
1993年论文
@zh-cn
name
Efficient random mutagenesis m ...... quency by use of PCR and dITP.
@en
type
label
Efficient random mutagenesis m ...... quency by use of PCR and dITP.
@en
prefLabel
Efficient random mutagenesis m ...... quency by use of PCR and dITP.
@en
P2093
P2860
P356
P1476
Efficient random mutagenesis m ...... quency by use of PCR and dITP.
@en
P2093
P2860
P304
P356
10.1093/NAR/21.3.777
P577
1993-02-01T00:00:00Z