Rapid and efficient construction of markerless deletions in the Escherichia coli genome - Wikidata - awgldk - TriplyDB

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

http://www.wikidata.org/entity/Q41727693

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A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette.High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage Site-specific chromosomal integration of large synthetic constructs.Rapid and highly efficient method for scarless mutagenesis within the Salmonella enterica chromosome.Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida.FRUIT, a scar-free system for targeted chromosomal mutagenesis, epitope tagging, and promoter replacement in Escherichia coli and Salmonella enterica.The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.A place for everything: chromosomal integration of large constructs.A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.Selective advantage of resistant strains at trace levels of antibiotics: a simple and ultrasensitive color test for detection of antibiotics and genotoxic agents.Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system On the road to synthetic life: the minimal cell and genome-scale engineering.Rapid prototyping of microbial cell factories via genome-scale engineering.Layering genetic circuits to build a single cell, bacterial half adder Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.An Integrated System for Precise Genome Modification in Escherichia coli PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli.Improved adhesive properties of recombinant bifidobacteria expressing the Bifidobacterium bifidum-specific lipoprotein BopA Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction.Scarless deletion of up to seven methyl-accepting chemotaxis genes with an optimized method highlights key function of CheM in Salmonella Typhimurium.CRMAGE: CRISPR Optimized MAGE Recombineering.Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci.Genome engineering Escherichia coli for L-DOPA overproduction from glucose Inducible Prophage Mutant of Escherichia coli Can Lyse New Host and the Key Sites of Receptor Recognition Identification.Development of Genetic Tools for the Manipulation of the Planctomycetes.Gene replacement techniques for Escherichia coli genome modification.Genome engineering and gene expression control for bacterial strain development.Strategies for cloning and manipulating natural and synthetic chromosomes.Molecular scissors and their application in genetically modified farm animals.Tandem repeat coupled with endonuclease cleavage (TREC): a seamless modification tool for genome engineering in yeast.Tough nuts to crack: site-directed mutagenesis of bifidobacteria remains a challenge.An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome.Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination.Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei.Multiple-site genetic modifications in Escherichia coli using lambda-Red recombination and I-SceI cleavage.Markerless Deletion System for Escherichia coli Using Short Homologous Sequences and Positive-Negative Selectable Cassette.Enhancing recombinant protein production with an Escherichia coli host strain lacking insertion sequences.Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440 Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producer A Multiplex Genome Editing Method for Based on CRISPR-Cas12a

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Rapid and efficient construction of markerless deletions in the Escherichia coli genome

http://www.wikidata.org/entity/Q41727693

description

2008 nî lūn-bûn

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2008年の論文

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2008年論文

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2008年論文

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2008年論文

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2008年論文

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2008年論文

@zh-tw

2008年论文

@wuu

2008年论文

@zh

2008年论文

@zh-cn

name

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

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type

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Rapid and efficient construction of markerless deletions in the Escherichia coli genome

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prefLabel

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

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Nucleic Acids Research

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Rapid and efficient construction of markerless deletions in the Escherichia coli genome

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P2093

Bong Hyun Sung

http://www.w3.org/2001/XMLSchema#string

Byung Jo Yu

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Jun Hyoung Lee

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Kui Hyeon Kang

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Mi Sun Kim

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Sun Chang Kim

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P2860

Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae

Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

Engineering a reduced Escherichia coli genome

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

Environmentally controlled invasion of cancer cells by engineered bacteria

Arac/XylS family of transcriptional regulators

An efficient recombination system for chromosome engineering in Escherichia coli

A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA

A new logic for DNA engineering using recombination in Escherichia coli

Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome.

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e84

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10.1093/NAR/GKN359

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14

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36

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