An economical method for production of (2)H, (13)CH3-threonine for solution NMR studies of large protein complexes: application to the 670 kDa proteasome.
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Structural Basis for Protein Antiaggregation Activity of the Trigger Factor ChaperoneFacilitating unambiguous NMR assignments and enabling higher probe density through selective labeling of all methyl containing amino acids(13)CHD2-CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins.Protein dynamics in living cells studied by in-cell NMR spectroscopy.Determination of ligand binding modes in weak protein-ligand complexes using sparse NMR data.Flexible stoichiometry and asymmetry of the PIDDosome core complex by heteronuclear NMR spectroscopy and mass spectrometry.Increased resolution of aromatic cross peaks using alternate 13C labeling and TROSY.Tracing an allosteric pathway regulating the activity of the HslV protease.NMR methods for structural studies of large monomeric and multimeric proteins.Practical aspects of NMR signal assignment in larger and challenging proteins.A Semiautomated Assignment Protocol for Methyl Group Side Chains in Large Proteins.Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.Exploiting E. coli auxotrophs for leucine, valine, and threonine specific methyl labeling of large proteins for NMR applications.Divide and conquer is always best: sensitivity of methyl correlation experiments.Specific labeling and assignment strategies of valine methyl groups for NMR studies of high molecular weight proteins.Selective labeling and unlabeling strategies in protein solid-state NMR spectroscopy.Automatic methyl assignment in large proteins by the MAGIC algorithm.Probing the cooperativity of Thermoplasma acidophilum proteasome core particle gating by NMR spectroscopy.Current NMR Techniques for Structure-Based Drug Discovery.Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and Other Prospective Peripheral Membrane Proteins.Detection and characterization of serine and threonine hydroxyl protons in Bacillus circulans xylanase by NMR spectroscopy.Differential isotope-labeling for Leu and Val residues in a protein by E. coli cellular expression using stereo-specifically methyl labeled amino acids.Late metabolic precursors for selective aromatic residue labelingPerspectives of solution NMR spectroscopy for structural and functional studies of integral membrane proteins
P2860
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P2860
An economical method for production of (2)H, (13)CH3-threonine for solution NMR studies of large protein complexes: application to the 670 kDa proteasome.
description
2012 nî lūn-bûn
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2012年の論文
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2012年学术文章
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name
An economical method for produ ...... ion to the 670 kDa proteasome.
@en
An economical method for produ ...... ion to the 670 kDa proteasome.
@nl
type
label
An economical method for produ ...... ion to the 670 kDa proteasome.
@en
An economical method for produ ...... ion to the 670 kDa proteasome.
@nl
prefLabel
An economical method for produ ...... ion to the 670 kDa proteasome.
@en
An economical method for produ ...... ion to the 670 kDa proteasome.
@nl
P2860
P1433
P1476
An economical method for produ ...... ion to the 670 kDa proteasome.
@en
P2093
Algirdas Velyvis
Amy M Ruschak
P2860
P304
P356
10.1371/JOURNAL.PONE.0043725
P407
P50
P577
2012-09-11T00:00:00Z