Gene optimization mechanisms: a multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli.
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Decoding mechanisms by which silent codon changes influence protein biogenesis and functionA ribosomal misincorporation of Lys for Arg in human triosephosphate isomerase expressed in Escherichia coli gives rise to two protein populationsGood codons, bad transcript: large reductions in gene expression and fitness arising from synonymous mutations in a key enzymeMultiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expressionSynonymous but not the same: the causes and consequences of codon biasCBDB: the codon bias database.Embedding permanent watermarks in synthetic genes.Sensitive measurement of single-nucleotide polymorphism-induced changes of RNA conformation: application to disease studies.Production of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli.A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiae.Hot or not? Discovery and characterization of a thermostable alditol oxidase from Acidothermus cellulolyticus 11B.Design, Construction and Cloning of Truncated ORF2 and tPAsp-PADRE-Truncated ORF2 Gene Cassette From Hepatitis E Virus in the pVAX1 Expression Vector.Codon Bias as a Means to Fine-Tune Gene Expression.Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production.Characterization of human paraoxonase 1 variants suggest that His residues at 115 and 134 positions are not always needed for the lactonase/arylesterase activities of the enzyme.Optimizing plant transporter expression in Xenopus oocytes.Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: advantages of high-throughput screening.Manipulating the genetic code for membrane protein production: what have we learnt so far?Co-expression for intracellular processing in microbial protein production.Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi.CpG domains downstream of TSSs promote high levels of gene expression.Codon Optimization Leads to Functional Impairment of RD114-TR Envelope Glycoprotein.Tools to cope with difficult-to-express proteins.Recent advances in (therapeutic protein) drug development.Stable In Vivo Transgene Expression in Endothelial Cells with Helper-Dependent Adenovirus: Roles of Promoter and Interleukin-10.Prediction of ribosome footprint profile shapes from transcript sequences.Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms.A new and updated resource for codon usage tablesMeasurement of average decoding rates of the 61 sense codons in vivo.Codon modification for the DNA sequence of a single-chain Fv antibody against clenbuterol and expression in Pichia pastoris.Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies.Improved production of membrane proteins in Escherichia coli by selective codon substitutions.
P2860
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P2860
Gene optimization mechanisms: a multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli.
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2010 nî lūn-bûn
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2010年の論文
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2010年論文
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2010年論文
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2010年論文
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2010年論文
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name
Gene optimization mechanisms: ...... expressed in Escherichia coli.
@en
Gene optimization mechanisms: ...... expressed in Escherichia coli.
@nl
type
label
Gene optimization mechanisms: ...... expressed in Escherichia coli.
@en
Gene optimization mechanisms: ...... expressed in Escherichia coli.
@nl
prefLabel
Gene optimization mechanisms: ...... expressed in Escherichia coli.
@en
Gene optimization mechanisms: ...... expressed in Escherichia coli.
@nl
P2093
P2860
P356
P1433
P1476
Gene optimization mechanisms: ...... expressed in Escherichia coli.
@en
P2093
Anne Spriestersbach
Barbara Maertens
Daniela Daubert
Frank Schäfer
Jan Kubicek
Marcus Graf
Michael Gerrits
Michael Liss
Ralf Wagner
P2860
P304
P356
10.1002/PRO.408
P577
2010-07-01T00:00:00Z