about
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolutionAn algorithm for protein engineering: simulations of recursive ensemble mutagenesisStructural Insights into the Evolution of a Non-Biological Protein: Importance of Surface Residues in Protein Fold OptimizationMutations that define the optimal half-site for binding yeast GCN4 activator protein and identify an ATF/CREB-like repressor that recognizes similar DNA sitesScoring functions for computational algorithms applicable to the design of spiked oligonucleotides.Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution.An algorithmically optimized combinatorial library screened by digital imaging spectroscopy.Glucocorticoid receptor-binding site in the human immunodeficiency virus long terminal repeat.The leucine zipper symmetrically positions the adjacent basic regions for specific DNA binding.Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactionsApplication of sorting and next generation sequencing to study 5΄-UTR influence on translation efficiency in Escherichia coliSaturation mutagenesis of a yeast his3 "TATA element": genetic evidence for a specific TATA-binding proteinIdentifying determinants of folding and activity for a protein of unknown structure.Mutational analysis of ligand binding activity of beta 2 adrenergic receptor expressed in Escherichia coliMutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1Enhancing the thermal robustness of an enzyme by directed evolution: least favorable starting points and inferior mutants can map superior evolutionary pathways.Mixed oligonucleotides for random mutagenesis: best way of making them.An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase.OVEC, a versatile system to study transcription in mammalian cells and cell-free extractsDeterminants of half-site spacing preferences that distinguish AP-1 and ATF/CREB bZIP domainsDimerization of leucine zippers analyzed by random selection.Identification of functional cis-regulatory elements by sequential enrichment from a randomized synthetic DNA library.Folded proteins occur frequently in libraries of random amino acid sequencesRecombination-dependent DNA replication stimulated by double-strand breaks in bacteriophage T4.U4B snRNA gene enhancer activity requires functional octamer and SPH motifs.Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae.Defining the consensus sequences of E.coli promoter elements by random selection.Critical sequences in the core of the P1 plasmid replication origin.Transcription of a bacteriophage lambda DNA site blocks growth of Escherichia coliDemonstration, characterization, and mutational analysis of NahR protein binding to nah and sal promoters.Saturation mutagenesis of an Escherichia coli rRNA promoter and initial characterization of promoter variants.Cooperatively folded proteins in random sequence libraries.Octamer and SPH motifs in the U1 enhancer cooperate to activate U1 RNA gene expressionA nucleosome-positioning sequence is required for GCN4 to activate transcription in the absence of a TATA element.Tc, an unusual promoter element required for constitutive transcription of the yeast HIS3 gene.Highly conserved residues in the bZIP domain of yeast GCN4 are not essential for DNA bindingUse of yeast nuclear DNA sequences to define the mitochondrial RNA polymerase promoter in vitroDefining the sequence specificity of DNA-binding proteins by selecting binding sites from random-sequence oligonucleotides: analysis of yeast GCN4 protein.Functional distinctions between yeast TATA elements.
P2860
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P2860
description
1986 nî lūn-bûn
@nan
1986年の論文
@ja
1986年学术文章
@wuu
1986年学术文章
@zh
1986年学术文章
@zh-cn
1986年学术文章
@zh-hans
1986年学术文章
@zh-my
1986年学术文章
@zh-sg
1986年學術文章
@yue
1986年學術文章
@zh-hant
name
Cloning of random-sequence oligodeoxynucleotides.
@en
Cloning of random-sequence oligodeoxynucleotides.
@nl
type
label
Cloning of random-sequence oligodeoxynucleotides.
@en
Cloning of random-sequence oligodeoxynucleotides.
@nl
prefLabel
Cloning of random-sequence oligodeoxynucleotides.
@en
Cloning of random-sequence oligodeoxynucleotides.
@nl
P2093
P1433
P1476
Cloning of random-sequence oligodeoxynucleotides.
@en
P2093
A L Nussbaum
A R Oliphant
P304
P356
10.1016/0378-1119(86)90180-0
P407
P577
1986-01-01T00:00:00Z