An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase.
about
Three decades of beta-lactamase inhibitorsViewing protein fitness landscapes through a next-gen lens.Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution.Selection and characterization of beta-lactam-beta-lactamase inactivator-resistant mutants following PCR mutagenesis of the TEM-1 beta-lactamase genePeptides on phage: a vast library of peptides for identifying ligandsApplying neutral drift to the directed molecular evolution of a β-glucuronidase into a β-galactosidase: Two different evolutionary pathways lead to the same variant.Mutational analysis of ligand binding activity of beta 2 adrenergic receptor expressed in Escherichia coliProtein engineering 20 years on.Single amino acid replacements at positions altered in naturally occurring extended-spectrum TEM beta-lactamases.Molecular characterization of nine different types of mutants among 107 inhibitor-resistant TEM beta-lactamases from clinical isolates of Escherichia coliIncidence and mechanisms of resistance to the combination of amoxicillin and clavulanic acid in Escherichia coli.First characterization of inhibitor-resistant TEM (IRT) beta-lactamases in Klebsiella pneumoniae strainsExtended-spectrum and inhibitor-resistant TEM-type beta-lactamases: mutations, specificity, and three-dimensional structure.Implication of Ile-69 and Thr-182 residues in kinetic characteristics of IRT-3 (TEM-32) beta-lactamaseProperties of IRT-14 (TEM-45), a newly characterized mutant of TEM-type beta-lactamases.A complex mutant of TEM-1 beta-lactamase with mutations encountered in both IRT-4 and extended-spectrum TEM-15, produced by an Escherichia coli clinical isolate.Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme.Yeast activator proteins and stress response: an overview.Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-1 beta-lactamase conferring resistance to beta-lactamase inhibitors.Characterization of a new TEM-type beta-lactamase resistant to clavulanate, sulbactam, and tazobactam in a clinical isolate of Escherichia coli.A genetic approach to the generation of antibodies with enhanced catalytic activitiesThymidine kinase mutants obtained by random sequence selection.Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide.Combinatorial libraries by cassette mutagenesis.Structure-function of muscarinic receptor coupling to G proteins. Random saturation mutagenesis identifies a critical determinant of receptor affinity for G proteins.Structure of a G-protein-coupling domain of a muscarinic receptor predicted by random saturation mutagenesis.Novel human DNA alkyltransferases obtained by random substitution and genetic selection in bacteriaExploring the active site of chorismate mutase by combinatorial mutagenesis and selection: the importance of electrostatic catalysis.Selection of naturally occurring extended-spectrum TEM beta-lactamase variants by fluctuating beta-lactam pressure.Combinatorial mutagenesis of the lamB gene: residues 41 through 43, which are conserved in Escherichia coli outer membrane proteins, are informationally important in maltoporin structure and function.Catalytic properties of class A beta-lactamases: efficiency and diversity.Thirty-degree shift in optimum temperature of a thermophilic lipase by a single-point mutation: effect of serine to threonine mutation on structural flexibility.SELEX: How It Happened and Where It will Go.Strategy for the directed evolution of a peptide ligase.
P2860
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P2860
An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase.
description
1989 nî lūn-bûn
@nan
1989 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած
@hyw
1989 թվականի դեկտեմբերին հրատարակված գիտական հոդված
@hy
1989年の論文
@ja
1989年論文
@yue
1989年論文
@zh-hant
1989年論文
@zh-hk
1989年論文
@zh-mo
1989年論文
@zh-tw
1989年论文
@wuu
name
An efficient method for genera ...... application to beta-lactamase.
@ast
An efficient method for genera ...... application to beta-lactamase.
@en
An efficient method for genera ...... application to beta-lactamase.
@nl
type
label
An efficient method for genera ...... application to beta-lactamase.
@ast
An efficient method for genera ...... application to beta-lactamase.
@en
An efficient method for genera ...... application to beta-lactamase.
@nl
prefLabel
An efficient method for genera ...... application to beta-lactamase.
@ast
An efficient method for genera ...... application to beta-lactamase.
@en
An efficient method for genera ...... application to beta-lactamase.
@nl
P2860
P356
P1476
An efficient method for genera ...... application to beta-lactamase.
@en
P2093
A R Oliphant
P2860
P304
P356
10.1073/PNAS.86.23.9094
P407
P577
1989-12-01T00:00:00Z