High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy.
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Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review.Process technology for production and recovery of heterologous proteins with Pichia pastoris.Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115.Potential of Ophiostoma piceae sterol esterase for biotechnologically relevant hydrolysis reactions.Beyond de-foaming: the effects of antifoams on bioprocess productivity.Effects of co-overexpression of secretion helper factors on the secretion of a HSA fusion protein (IL2-HSA) in Pichia pastoris.A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains.Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter.A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastorisFunctional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris.Fast development of Pichia pastoris GS115 Mut(+) cultures employing batch-to-batch control and hybrid semi-parametric modeling.Maximization of production of secreted recombinant proteins in Pichia pastoris fed-batch fermentation.Pichia pastoris fermentation optimization: energy state and testing a growth-associated model.Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pastoris.Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by Komagataella (Pichia) pastoris.Design of a novel automated methanol feed system for pilot-scale fermentation of Pichia pastoris.Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation.Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut+ strain.A macrokinetic model-based comparative meta-analysis of recombinant protein production by Pichia pastoris under AOX1 promoter.Evaluation of metals in a defined medium for Pichia pastoris expressing recombinant beta-galactosidase.Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris.A simple model-based control for Pichia pastoris allows a more efficient heterologous protein production bioprocess.Application of adaptive DO-stat feeding control to Pichia pastoris X33 cultures expressing a single chain antibody fragment (scFv).Production of a sterol esterase from Ophiostoma piceae in batch and fed-batch bioprocesses using different Pichia pastoris phenotypes as cell factory.Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity.
P2860
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P2860
High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy.
description
2000 nî lūn-bûn
@nan
2000年の論文
@ja
2000年学术文章
@wuu
2000年学术文章
@zh
2000年学术文章
@zh-cn
2000年学术文章
@zh-hans
2000年学术文章
@zh-my
2000年学术文章
@zh-sg
2000年學術文章
@yue
2000年學術文章
@zh-hant
name
High level secretion of recomb ...... mal methanol feeding strategy.
@en
High level secretion of recomb ...... mal methanol feeding strategy.
@nl
type
label
High level secretion of recomb ...... mal methanol feeding strategy.
@en
High level secretion of recomb ...... mal methanol feeding strategy.
@nl
prefLabel
High level secretion of recomb ...... mal methanol feeding strategy.
@en
High level secretion of recomb ...... mal methanol feeding strategy.
@nl
P2093
P1476
High level secretion of recomb ...... imal methanol feeding strategy
@en
P2093
P304
P356
10.1016/S1389-1723(00)80082-1
P577
2000-01-01T00:00:00Z