Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.
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Nature's recipe for splitting inteinsCharacterization of Molecular Interactions between ACP and Halogenase Domains in the Curacin A Polyketide SynthaseNMR and Crystal Structures of the Pyrococcus horikoshii RadA Intein Guide a Strategy for Engineering a Highly Efficient and Promiscuous InteinStructure-based engineering and comparison of novel split inteins for protein ligationNMR approaches in structure-based lead discovery: recent developments and new frontiers for targeting multi-protein complexesProtein trans-splicing as a protein ligation tool to study protein structure and function.Use of protein trans-splicing to produce active and segmentally (2)H, (15)N labeled mannuronan C5-epimerase AlgE4Prediction of peak overlap in NMR spectra.Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register.Recent progress in intein research: from mechanism to directed evolution and applications.Circular permutation prediction reveals a viable backbone disconnection for split proteins: an approach in identifying a new functional split intein.SPLICEFINDER - a fast and easy screening method for active protein trans-splicing positions.Highly efficient and more general cis- and trans-splicing inteins through sequential directed evolution.Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split inteinSegmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing.Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering.Extein residues play an intimate role in the rate-limiting step of protein trans-splicing.Split Inteins: Nature's Protein Ligases.Characterizing Aciniform Silk Repetitive Domain Backbone Dynamics and Hydrodynamic Modularity.Structural analysis of protein-protein interactions in type I polyketide synthasesSAIL--stereo-array isotope labeling.Split-inteins and their bioapplications.Split-inteins for simultaneous, site-specific conjugation of quantum dots to multiple protein targets in vivo.Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing.'Traceless' tracing of proteins - high-affinity trans-splicing directed by a minimal interaction pair.Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources.Hideo Iwai. Interview by Daniela Ruffell.Optimization of amino acid type-specific 13C and 15N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm.Split intein mediated ultra-rapid purification of tagless protein (SIRP).Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro.
P2860
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P2860
Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.
description
2009 nî lūn-bûn
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2009年の論文
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2009年学术文章
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2009年学术文章
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2009年学术文章
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name
Segmental isotopic labeling of ...... g only one robust DnaE intein.
@en
Segmental isotopic labeling of ...... g only one robust DnaE intein.
@nl
type
label
Segmental isotopic labeling of ...... g only one robust DnaE intein.
@en
Segmental isotopic labeling of ...... g only one robust DnaE intein.
@nl
prefLabel
Segmental isotopic labeling of ...... g only one robust DnaE intein.
@en
Segmental isotopic labeling of ...... g only one robust DnaE intein.
@nl
P2093
P50
P356
P1476
Segmental isotopic labeling of ...... g only one robust DnaE intein.
@en
P2093
A Sesilja Aranko
Alena E L Busche
Mehdi Talebzadeh-Farooji
P304
P356
10.1002/ANIE.200901488
P407
P577
2009-01-01T00:00:00Z