Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis
about
Human cyclophilin B: a second cyclophilin gene encodes a peptidyl-prolyl isomerase with a signal sequenceA novel FK506 binding protein can mediate the immunosuppressive effects of FK506 and is associated with the cardiac ryanodine receptorCrystal structure of human calcineurin complexed with cyclosporin A and human cyclophilinp-Aminobenzoate synthesis in Escherichia coli: purification and characterization of PabB as aminodeoxychorismate synthase and enzyme X as aminodeoxychorismate lyaseAn endoplasmic reticulum-specific cyclophilinThe X-ray structure of a divergent cyclophilin from the nematode parasite Brugia malayiSynthesis and structural analysis of 2-quinuclidonium tetrafluoroborateSolution structure of FKBP, a rotamase enzyme and receptor for FK506 and rapamycinNative recombinant cyclophilins A, B, and C degrade DNA independently of peptidylprolyl cis-trans-isomerase activity. Potential roles of cyclophilins in apoptosisChaperone-like activity of peptidyl-prolyl cis-trans isomerase during creatine kinase refoldingSevere osteogenesis imperfecta in cyclophilin B-deficient miceDetailed characterization of a cyclophilin from the human malaria parasite Plasmodium falciparumCrystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolutionKinetic isotope effects support the twisted amide mechanism of Pin1 peptidyl-prolyl isomeraseAntibody catalysis of peptidyl-prolyl cis-trans isomerization in the folding of RNase T1.Cyclophilin a binds to peroxiredoxins and activates its peroxidase activity.Structure and expression of cytosolic cyclophilin/peptidyl-prolyl cis-trans isomerase of higher plants and production of active tomato cyclophilin in Escherichia coli.Throughput and efficiency of a mass spectrometry-based screening assay for protein-ligand binding detection.Use of a real-time fluorescence monitoring system for high-throughput screening for prolyl isomerase inhibitors.Hydrogen/deuterium exchange- and protease digestion-based screening assay for protein-ligand binding detection.Peptidyl-prolyl cis-trans-isomerase from Escherichia coli: a periplasmic homolog of cyclophilin that is not inhibited by cyclosporin A.Discovery of novel cyclophilin A ligands using an H/D exchange- and mass spectrometry-based strategy.The intriguing cyclophilin A-HIV-1 Vpr interaction: prolyl cis/trans isomerisation catalysis and specific binding.StableIsotope Labeling with Amino Acids in Cell Culture (SILAC)-based strategy for proteome-wide thermodynamic analysis of protein-ligand binding interactions.Solvent Effects on the Energetics of Prolyl Peptide Bond IsomerizationQuantitative proteomics approach for identifying protein-drug interactions in complex mixtures using protein stability measurements.Structural characterization of N-protonated amides: regioselective N-activation of medium-bridged twisted lactamsImplication of cysteine residues in the selection of oxorhenium inhibitors of cyclophilin hCyp18.Chemistry of bridged lactams and related heterocyclesImmunophilins: structure-function relationship and possible role in microbial pathogenicity.Retina-specifically expressed novel subtypes of bovine cyclophilin.Stable isotope labeling strategy for protein-ligand binding analysis in multi-component protein mixtures.Expression of recombinant human casein kinase II and recombinant heat shock protein 90 in Escherichia coli and characterization of their interactionsEnhancing the stability and folding rate of a repeat protein through the addition of consensus repeatsCrystal structure of cyclophilin A complexed with substrate Ala-Pro suggests a solvent-assisted mechanism of cis-trans isomerizationDelineation of the calcineurin-interacting region of cyclophilin B.Thermo-kinetic analysis space expansion for cyclophilin-ligand interactions - identification of a new nonpeptide inhibitor using Biacore™ T200.Overexpression of OsCYP19-4 increases tolerance to cold stress and enhances grain yield in rice (Oryza sativa)GroEL-GroES-mediated protein folding requires an intact central cavity.Mechanistic insight into the role of transition-state stabilization in cyclophilin A.
P2860
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P2860
Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis
description
1990 nî lūn-bûn
@nan
1990 թուականի Մարտին հրատարակուած գիտական յօդուած
@hyw
1990 թվականի մարտին հրատարակված գիտական հոդված
@hy
1990年の論文
@ja
1990年論文
@yue
1990年論文
@zh-hant
1990年論文
@zh-hk
1990年論文
@zh-mo
1990年論文
@zh-tw
1990年论文
@wuu
name
Cloning, expression, and purif ...... s by site-directed mutagenesis
@ast
Cloning, expression, and purif ...... s by site-directed mutagenesis
@en
Cloning, expression, and purif ...... s by site-directed mutagenesis
@en-gb
Cloning, expression, and purif ...... s by site-directed mutagenesis
@nl
type
label
Cloning, expression, and purif ...... s by site-directed mutagenesis
@ast
Cloning, expression, and purif ...... s by site-directed mutagenesis
@en
Cloning, expression, and purif ...... s by site-directed mutagenesis
@en-gb
Cloning, expression, and purif ...... s by site-directed mutagenesis
@nl
prefLabel
Cloning, expression, and purif ...... s by site-directed mutagenesis
@ast
Cloning, expression, and purif ...... s by site-directed mutagenesis
@en
Cloning, expression, and purif ...... s by site-directed mutagenesis
@en-gb
Cloning, expression, and purif ...... s by site-directed mutagenesis
@nl
P2093
P2860
P921
P356
P1476
Cloning, expression, and purif ...... s by site-directed mutagenesis
@en
P2093
P2860
P304
P356
10.1073/PNAS.87.6.2304
P407
P577
1990-03-01T00:00:00Z