about
Fumarase: a mitochondrial metabolic enzyme and a cytosolic/nuclear component of the DNA damage responseEngineering proteinase K using machine learning and synthetic genesIGHMBP2 is a ribosome-associated helicase inactive in the neuromuscular disorder distal SMA type 1 (DSMA1)Putting synthesis into biology: a viral view of genetic engineering through de novo gene and genome synthesisCorrecting errors in synthetic DNA through consensus shuffling.Error correction in gene synthesis technologyA simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in pichiaRational Design of High-Number dsDNA Fragments Based on Thermodynamics for the Construction of Full-Length Genes in a Single ReactionThe effect of amino acid deletions and substitutions in the longest loop of GFPGene composer: database software for protein construct design, codon engineering, and gene synthesisRapid assembly of multiple-exon cDNA directly from genomic DNA.Novel method of cell-free in vitro synthesis of the human fibroblast growth factor 1 geneAlanine-shaving mutagenesis to determine key interfacial residues governing the assembly of a nano-cage maxi-ferritinFunctional hybrid rubisco enzymes with plant small subunits and algal large subunits: engineered rbcS cDNA for expression in chlamydomonas.Large-scale de novo DNA synthesis: technologies and applications.de novo design and synthesis of Candida antarctica lipase B gene and α-factor leads to high-level expression in Pichia pastoris.High-throughput cloning and expression library creation for functional proteomicsA quantitative model of error accumulation during PCR amplification.Mutant Prpf31 causes pre-mRNA splicing defects and rod photoreceptor cell degeneration in a zebrafish model for Retinitis pigmentosa.The exonuclease Nibbler regulates age-associated traits and modulates piRNA length in Drosophila.Error correction of microchip synthesized genes using Surveyor nuclease.Generation of infectious molecular clones of simian immunodeficiency virus from fecal consensus sequences of wild chimpanzeesNuclear expression of a mitochondrial DNA gene: mitochondrial targeting of allotopically expressed mutant ATP6 in transgenic mice.Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins.Efficient detection, quantification and enrichment of subtle allelic alterations.Protein fabrication automationSynthetic biology--putting engineering into biology.Multiplex pairwise assembly of array-derived DNA oligonucleotidesSimultaneous splicing of multiple DNA fragments in one PCR reactionEfficient transposition of Tol2 in the mouse germline.Construction of a novel liver-targeting fusion interferon by incorporation of a Plasmodium region I-plus peptide.Microbial proteases: detection, production, and genetic improvement.TmPrime: fast, flexible oligonucleotide design software for gene synthesis.Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis.Oral Rabies Vaccine Design for Expression in Plants.PCR-based gene synthesis to produce recombinant proteins for crystallization.Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.The synthesis and high-level expression of a beta2-adrenergic receptor gene in a tetracycline-inducible stable mammalian cell line.Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes.Study of immunogenicity of recombinant proteins based on hemagglutinin and neuraminidase conservative epitopes of influenza A virus
P2860
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P2860
description
2004 nî lūn-bûn
@nan
2004 թուականին հրատարակուած գիտական յօդուած
@hyw
2004 թվականին հրատարակված գիտական հոդված
@hy
2004年の論文
@ja
2004年論文
@yue
2004年論文
@zh-hant
2004年論文
@zh-hk
2004年論文
@zh-mo
2004年論文
@zh-tw
2004年论文
@wuu
name
Two-step total gene synthesis method
@ast
Two-step total gene synthesis method
@en
Two-step total gene synthesis method
@nl
type
label
Two-step total gene synthesis method
@ast
Two-step total gene synthesis method
@en
Two-step total gene synthesis method
@nl
prefLabel
Two-step total gene synthesis method
@ast
Two-step total gene synthesis method
@en
Two-step total gene synthesis method
@nl
P2860
P356
P1476
Two-step total gene synthesis method
@en
P2093
P2860
P356
10.1093/NAR/GNH058
P407
P577
2004-01-01T00:00:00Z