An efficient one-step site-directed and site-saturation mutagenesis protocol - Test2 - elhamkhani - TriplyDB

An efficient one-step site-directed and site-saturation mutagenesis protocol

An efficient one-step site-directed and site-saturation mutagenesis protocol

http://www.wikidata.org/entity/Q29614689

about

Hepatocyte permissiveness to Plasmodium infection is conveyed by a short and structurally conserved region of the CD81 large extracellular domain An essential role for maternal control of Nodal signaling Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment Phosphorylation of myofibrillogenesis regulator-1 activates the MAPK signaling pathway and induces proliferation and migration in human breast cancer MCF7 cells PARP16 is a tail-anchored endoplasmic reticulum protein required for the PERK- and IRE1α-mediated unfolded protein response The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function Adenylylation control by intra- or intermolecular active-site obstruction in Fic proteins Human ind1, an iron-sulfur cluster assembly factor for respiratory complex I MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity Mechanism of the Class I KDPG aldolase Molecular basis for the action of the collagen-specific chaperone Hsp47/SERPINH1 and its structure-specific client recognition Human perforin mutations and susceptibility to multiple primary cancers Feature Article: mTOR complex 2-Akt signaling at mitochondria-associated endoplasmic reticulum membranes (MAM) regulates mitochondrial physiology Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design Crystal structure of the yeast eIF4A-eIF4G complex: an RNA-helicase controlled by protein-protein interactions Rational Protein Engineering Guided by Deep Mutational Scanning Mathematical and live meningococcal models for simple sequence repeat dynamics - coherent predictions and observations Discrimination between Closely Related Cellular Metabolites by the SAM-I Riboswitch A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens Characterization and crystal structure of Escherichia coli KDPGal aldolase Sialic acid mutarotation is catalyzed by the Escherichia coli beta-propeller protein YjhT Substrate binding, deprotonation, and selectivity at the periplasmic entrance of the Escherichia coli ammonia channel AmtB Structural Variations in Species B Adenovirus Fibers Impact CD46 Association Analysis of a Critical Interaction within the Archaeal Box C/D Small Ribonucleoprotein Complex The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes Active site remodeling switches HIV specificity of antiretroviral TRIMCyp The crystal structure of apo-FtsH reveals domain movements necessary for substrate unfolding and translocation.Structural and Kinetic Analysis ofSchwanniomyces occidentalisInvertase Reveals a New Oligomerization Pattern and the Role of Its Supplementary Domain in Substrate Binding Features critical for membrane binding revealed by DivIVA crystal structure Conformational Adaptation of Asian Macaque TRIMCyp Directs Lineage Specific Antiviral Activity A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop Compensating Stereochemical Changes Allow Murein Tripeptide to Be Accommodated in a Conventional Peptide-binding Protein Crystal Structures of Archaemetzincin Reveal a Moldable Substrate-Binding Site A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage Crystal Structure of the Klebsiella pneumoniae NFeoB/FeoC Complex and Roles of FeoC in Regulation of Fe2+ Transport by the Bacterial Feo System Crystal Structure of Bovine Coronavirus Spike Protein Lectin Domain Evolution to carbapenem-hydrolyzing activity in noncarbapenemase class D -lactamase OXA-10 by rational protein design Facile backbone structure determination of human membrane proteins by NMR spectroscopy

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P2860

An efficient one-step site-directed and site-saturation mutagenesis protocol

http://www.wikidata.org/entity/Q29614689

description

2004 nî lūn-bûn

@nan

2004 թուականի Օգոստոսին հրատարակուած գիտական յօդուած

@hyw

2004 թվականի օգոստոսին հրատարակված գիտական հոդված

@hy

2004年の論文

@ja

2004年論文

@yue

2004年論文

@zh-hant

2004年論文

@zh-hk

2004年論文

@zh-mo

2004年論文

@zh-tw

2004年论文

@wuu

name

An efficient one-step site-directed and site-saturation mutagenesis protocol

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An efficient one-step site-directed and site-saturation mutagenesis protocol

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type

label

An efficient one-step site-directed and site-saturation mutagenesis protocol

@ast

An efficient one-step site-directed and site-saturation mutagenesis protocol

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prefLabel

An efficient one-step site-directed and site-saturation mutagenesis protocol

@ast

An efficient one-step site-directed and site-saturation mutagenesis protocol

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Nucleic Acids Research

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An efficient one-step site-directed and site-saturation mutagenesis protocol

@en

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Baumann U

http://www.w3.org/2001/XMLSchema#string

Reymond JL

http://www.w3.org/2001/XMLSchema#string

Zheng L

http://www.w3.org/2001/XMLSchema#string

P2860

Directed evolution of N-acetylneuraminic acid aldolase to catalyze enantiomeric aldol reactions.

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function.

Directed evolution study of temperature adaptation in a psychrophilic enzyme.

Approaches to DNA mutagenesis: an overview.

Molecular mechanism of enantioselective proton transfer to carbon in catalytic antibody 14D9

Site-directed mutagenesis using overlap extension PCR.

Production of a functional catalytic antibody ScFv-NusA fusion protein in bacterial cytoplasm.

Expression improvement and mechanistic study of the retro-Diels-Alderase catalytic antibody 10F11 by site-directed mutagenesis.

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e115

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scholarly article

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348531

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10.1093/NAR/GNH110

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14

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32

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8405820

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15304544

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514394

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