An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.
about
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studiesStructure-function features of a Mycoplasma glycolipid synthase derived from structural data integration, molecular simulations, and mutational analysisDirect monitoring of the strand passage reaction of DNA topoisomerase II triggers checkpoint activationStructural Basis for Acyl Acceptor Specificity in the Achromobactin Biosynthetic Enzyme AcsDStructure of WbdD: a bifunctional kinase and methyltransferase that regulates the chain length of the O antigen inEscherichia coliO9aDisplacement of the canonical single-stranded DNA-binding protein in the ThermoprotealesA conserved asparagine has a structural role in ubiquitin-conjugating enzymesCrystal Structures of the Sec1/Munc18 (SM) Protein Vps33, Alone and Bound to the Homotypic Fusion and Vacuolar Protein Sorting (HOPS) Subunit Vps16*Structure of the archaeal Cascade subunit Csa5Crystal Structure of the Heterodimeric CLOCK:BMAL1 Transcriptional Activator ComplexA monoclinic crystal form of casein kinase 1 δSolution Structure, Membrane Interactions, and Protein Binding Partners of the Tetraspanin Sm-TSP-2, a Vaccine Antigen from the Human Blood FlukeSchistosoma mansoniHigh-Resolution Structure of the N-Terminal Endonuclease Domain of the Lassa Virus L Polymerase in Complex with Magnesium IonsStructural basis of Ets1 activation by Runx1Structural basis for binding the TREX2 complex to nuclear pores, GAL1 localisation and mRNA exportRegulation of ubiquitin transfer by XIAP, a dimeric RING E3 ligaseBacterial actin MreB forms antiparallel double filamentsHigh-resolution crystal structure of spin labelled (T21R1) azurin from Pseudomonas aeruginosa: a challenging structural benchmark for in silico spin labelling algorithmsElucidation of the Molecular Basis for Arabinoxylan-Debranching Activity of a Thermostable Family GH62 -L-Arabinofuranosidase from Streptomyces thermoviolaceusProduction of Fatty Acid-derived valuable chemicals in synthetic microbesStrategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expressionDNA polymerase δ and ζ switch by sharing accessory subunits of DNA polymerase δSympathetic nervous system overactivity and its role in the development of cardiovascular diseaseStructural insights into cardiolipin transfer from the Inner membrane to the outer membrane by PbgA in Gram-negative bacteriaOne-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution CollectionsMost neutralizing human monoclonal antibodies target novel epitopes requiring both Lassa virus glycoprotein subunitsThe RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexesSensitization of TRPA1 by Protein Kinase AGenome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine ProductionA novel fatty acid-binding protein-like carotenoid-binding protein from the gonad of the New Zealand sea urchin Evechinus chloroticus.Regulated membrane remodeling by Mic60 controls formation of mitochondrial crista junctionsAAscan, PCRdesign and MutantChecker: a suite of programs for primer design and sequence analysis for high-throughput scanning mutagenesis.A novel chromatin tether domain controls topoisomerase IIα dynamics and mitotic chromosome formation.Long-range allosteric signaling in red light-regulated diguanylyl cyclases.Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates.Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9.Multichange isothermal mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA.A piggyBac-based reporter system for scalable in vitro and in vivo analysis of 3' untranslated region-mediated gene regulationTcf3 promotes cell migration and wound repair through regulation of lipocalin 2.Astrocyte chemoreceptors: mechanisms of H+ sensing by astrocytes in the retrotrapezoid nucleus and their possible contribution to respiratory drive.
P2860
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P2860
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.
description
2008 nî lūn-bûn
@nan
2008 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2008 թվականի դեկտեմբերին հրատարակված գիտական հոդված
@hy
2008年の論文
@ja
2008年論文
@yue
2008年論文
@zh-hant
2008年論文
@zh-hk
2008年論文
@zh-mo
2008年論文
@zh-tw
2008年论文
@wuu
name
An efficient one-step site-dir ...... e plasmid mutagenesis protocol
@nl
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@ast
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@en
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@en-gb
type
label
An efficient one-step site-dir ...... e plasmid mutagenesis protocol
@nl
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@ast
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@en
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@en-gb
prefLabel
An efficient one-step site-dir ...... e plasmid mutagenesis protocol
@nl
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@ast
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@en
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@en-gb
P2860
P356
P1433
P1476
An efficient one-step site-dir ...... plasmid mutagenesis protocol.
@en
P2093
Huanting Liu
P2860
P2888
P356
10.1186/1472-6750-8-49
P407
P50
P577
2008-12-04T00:00:00Z
P5875
P6179
1017252247