Alanine-scanning mutations in the "primer grip" of p66 HIV-1 reverse transcriptase result in selective loss of RNA priming activity.
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Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNAHIV-1 reverse transcriptionHIV-1 Reverse Transcriptase Structure with RNase H Inhibitor Dihydroxy Benzoyl Naphthyl Hydrazone Bound at a Novel SiteEffects of 3' untranslated region mutations on plus-strand priming during moloney murine leukemia virus replication.Mutants of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase resistant to nonnucleoside reverse transcriptase inhibitors demonstrate altered rates of RNase H cleavage that correlate with HIV-1 replication fitness in cell culture.Structure and function of HIV-1 reverse transcriptase: molecular mechanisms of polymerization and inhibition.Analysis of human immunodeficiency virus type 1 reverse transcriptase subunit structure/function in the context of infectious virions and human target cells.The N348I mutation at the connection subdomain of HIV-1 reverse transcriptase decreases binding to nevirapine.Mutations proximal to the minor groove-binding track of human immunodeficiency virus type 1 reverse transcriptase differentially affect utilization of RNA versus DNA as template.Human immunodeficiency virus type 2 reverse transcriptase activity in model systems that mimic steps in reverse transcription.Biochemical, inhibition and inhibitor resistance studies of xenotropic murine leukemia virus-related virus reverse transcriptase.Inhibition of HIV-1 reverse transcription: basic principles of drug action and resistance.Deaminase-independent inhibition of HIV-1 reverse transcription by APOBEC3G.Human immunodeficiency virus reverse transcriptase: 25 years of research, drug discovery, and promise.Fidelity of plus-strand priming requires the nucleic acid chaperone activity of HIV-1 nucleocapsid protein.Solution characterization of [methyl-(13)C]methionine HIV-1 reverse transcriptase by NMR spectroscopyUsing pyrrolo-deoxycytosine to probe RNA/DNA hybrids containing the human immunodeficiency virus type-1 3' polypurine tract.Two modes of HIV-1 polypurine tract cleavage are affected by introducing locked nucleic acid analogs into the (-) DNA template.Efficient initiation of HIV-1 reverse transcription in vitro. Requirement for RNA sequences downstream of the primer binding site abrogated by nucleocapsid protein-dependent primer-template interactions.The glutamine side chain at position 91 on the β5a-β5b loop of human immunodeficiency virus type 1 reverse transcriptase is required for stabilizing the dNTP binding pocket.A mutation in the primer grip region of HIV-1 reverse transcriptase that confers reduced fidelity of DNA synthesisSequence requirements for removal of tRNA by an isolated human immunodeficiency virus type 1 RNase H domainMutations in the primer grip of human immunodeficiency virus type 1 reverse transcriptase impair proviral DNA synthesis and virion maturation.Selection of optimal polypurine tract region sequences during Moloney murine leukemia virus replication.Comparison of second-strand transfer requirements and RNase H cleavages catalyzed by human immunodeficiency virus type 1 reverse transcriptase (RT) and E478Q RT.Second-site reversion of a human immunodeficiency virus type 1 reverse transcriptase mutant that restores enzyme function and replication capacity.RNase H requirements for the second strand transfer reaction of human immunodeficiency virus type 1 reverse transcription.The M230L nonnucleoside reverse transcriptase inhibitor resistance mutation in HIV-1 reverse transcriptase impairs enzymatic function and viral replicative capacity.Structural Aspects of Drug Resistance and Inhibition of HIV-1 Reverse Transcriptase.Increased G-->A transition frequencies displayed by primer grip mutants of human immunodeficiency virus type 1 reverse transcriptaseSeparation of protein oligomers by blue native gel electrophoresis.The sequential mechanism of HIV reverse transcriptase RNase H.
P2860
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P2860
Alanine-scanning mutations in the "primer grip" of p66 HIV-1 reverse transcriptase result in selective loss of RNA priming activity.
description
1997 nî lūn-bûn
@nan
1997年の論文
@ja
1997年論文
@yue
1997年論文
@zh-hant
1997年論文
@zh-hk
1997年論文
@zh-mo
1997年論文
@zh-tw
1997年论文
@wuu
1997年论文
@zh
1997年论文
@zh-cn
name
Alanine-scanning mutations in ...... loss of RNA priming activity.
@en
type
label
Alanine-scanning mutations in ...... loss of RNA priming activity.
@en
prefLabel
Alanine-scanning mutations in ...... loss of RNA priming activity.
@en
P2093
P2860
P356
P1476
Alanine-scanning mutations in ...... loss of RNA priming activity.
@en
P2093
P2860
P304
13262-13269
P356
10.1074/JBC.272.20.13262
P407
P577
1997-05-01T00:00:00Z