A simple method for site-directed mutagenesis using the polymerase chain reaction
about
Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotectionTranscription factor ZBP-89 regulates the activity of the ornithine decarboxylase promoterThe N-terminal 26-residue fragment of human programmed cell death 5 protein can form a stable alpha-helix having unique electrostatic potential characterRibonuclease E organizes the protein interactions in the Escherichia coli RNA degradosomeCOT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiaeTranscription factor GATA-4 regulates cardiac muscle-specific expression of the alpha-myosin heavy-chain geneAssociation of p62, a multifunctional SH2- and SH3-domain-binding protein, with src family tyrosine kinases, Grb2, and phospholipase C gamma-1Mutational analysis of the DNA binding, dimerization, and transcriptional activation domains of MEF2CDifferential 3' splice site recognition of SMN1 and SMN2 transcripts by U2AF and U2 snRNPBinding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteinsFour distinct pathways of hemoglobin uptake in the malaria parasite Plasmodium falciparum.Angiotensin II type1a receptor gene expression in the heart: AP-1 and GATA-4 participate in the response to pressure overloadSiteFind: a software tool for introducing a restriction site as a marker for successful site-directed mutagenesis.The NS2 protein of hepatitis C virus is a transmembrane polypeptideChimeric Sindbis viruses dependent on the NS3 protease of hepatitis C virusStructure of a protein G helix variant suggests the importance of helix propensity and helix dipole interactions in protein designFolding Stability and Cooperativity of the Three Forms of 1–110 Residues Fragment of Staphylococcal NucleaseStructural Evidence for Enhancement of Sequential Vitamin D3 Hydroxylation Activities by Directed Evolution of Cytochrome P450 Vitamin D3 HydroxylaseStructures of Burkholderia thailandensis nucleoside kinase: implications for the catalytic mechanism and nucleoside selectivityStructural and Functional Insights into Saccharomyces cerevisiae Riboflavin Biosynthesis Reductase RIB7Mechanistic Strategies for Catalysis Adopted by Evolutionary Distinct Family 43 ArabinanasesA single mutation at the ferredoxin binding site of P450 Vdh enables efficient biocatalytic production of 25-hydroxyvitamin D(3)Structural and functional characterization of a ruminal β-glycosidase defines a novel subfamily of glycosyl hydrolase family 3 with permuted domain topologyRiboflavin biosynthesis in Saccharomyces cerevisiae. Cloning, characterization, and expression of the RIB5 gene encoding riboflavin synthase.Role of nuclear pools of aminoacyl-tRNA synthetases in tRNA nuclear export.Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition.Actin-binding verprolin is a polarity development protein required for the morphogenesis and function of the yeast actin cytoskeleton.Nucleocytoplasmic shuttling of heterodimeric splicing factor U2AFNotch ligands are substrates for protein O-fucosyltransferase-1 and FringeHuman cathepsin H: deletion of the mini-chain switches substrate specificity from aminopeptidase to endopeptidaseThe conserved box 1 motif of cytokine receptors is required for association with JAK kinasesWW domains of Rsp5p define different functions: determination of roles in fluid phase and uracil permease endocytosis in Saccharomyces cerevisiaeThe Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates bio-mineralization proteinsParalogous chemoreceptors mediate chemotaxis towards protein amino acids and the non-protein amino acid gamma-aminobutyrate (GABA)Vfr controls quorum sensing in Pseudomonas aeruginosaThe cardiac tissue-restricted homeobox protein Csx/Nkx2.5 physically associates with the zinc finger protein GATA4 and cooperatively activates atrial natriuretic factor gene expression.MEF2B is a potent transactivator expressed in early myogenic lineagesRecombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studiesThe pCri System: a vector collection for recombinant protein expression and purificationStructural and Biochemical Insights into the Peptidoglycan Hydrolase Domain of FlgJ from Salmonella typhimurium
P2860
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P2860
A simple method for site-directed mutagenesis using the polymerase chain reaction
description
1989 nî lūn-bûn
@nan
1989年の論文
@ja
1989年論文
@yue
1989年論文
@zh-hant
1989年論文
@zh-hk
1989年論文
@zh-mo
1989年論文
@zh-tw
1989年论文
@wuu
1989年论文
@zh
1989年论文
@zh-cn
name
A simple method for site-directed mutagenesis using the polymerase chain reaction
@en
type
label
A simple method for site-directed mutagenesis using the polymerase chain reaction
@en
prefLabel
A simple method for site-directed mutagenesis using the polymerase chain reaction
@en
P2093
P2860
P356
P1476
A simple method for site-directed mutagenesis using the polymerase chain reaction
@en
P2093
P2860
P304
P356
10.1093/NAR/17.16.6545
P577
1989-08-01T00:00:00Z