about
The bud tip is the cellular hot spot of protein secretion in yeasts.A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastorisGenome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris.Open access to sequence: browsing the Pichia pastoris genome.Monitoring of transcriptional regulation in Pichia pastoris under protein production conditionsNovel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays.Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production in Pichia pastoris.The response to unfolded protein is involved in osmotolerance of Pichia pastoris.A multi-level study of recombinant Pichia pastoris in different oxygen conditions.Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems?Induction and measurement of UPR and osmotic stress in the yeast Pichia pastoris.Model based engineering of Pichia pastoris central metabolism enhances recombinant protein productionBiomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures.Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level.Systems-level organization of yeast methylotrophic lifestyle.Stress in recombinant protein producing yeasts.Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris.Functional inclusion bodies produced in the yeast Pichia pastoris.Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastorisUnconventional microbial systems for the cost-efficient production of high-quality protein therapeutics.Engineering of protein folding and secretion-strategies to overcome bottlenecks for efficient production of recombinant proteins.Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris.Yeast biotechnology: teaching the old dog new tricks.Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.Curation of the genome annotation of Pichia pastoris (Komagataella phaffii) CBS7435 from gene level to protein function.Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris.Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts.Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response.Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteinsPichia pastoris Aft1--a novel transcription factor, enhancing recombinant protein secretion.Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris.Recombinant protein production 6 (Vienna, February 2011).Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus.Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris.Identification and deletion of the major secreted protein of Pichia pastoris.Creation of stable heterothallic strains of Komagataella phaffii enables dissection of mating gene regulation.GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris.Systems biotechnology for protein production in Pichia pastoris.
P50
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P50
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hulumtuese
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հետազոտող
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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Brigitte Gasser
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