Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids
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Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion toolStructural basis for the site-specific incorporation of lysine derivatives into proteinsA novel crystal form of pyrrolysyl-tRNA synthetase reveals the pre- and post-aminoacyl-tRNA synthesis conformational states of the adenylate and aminoacyl moieties and an asparagine residue in the catalytic siteStructural basis of furan-amino acid recognition by a polyspecific aminoacyl-tRNA-synthetase and its genetic encoding in human cellsGenetically encoding photoswitchable click amino acids in Escherichia coli and mammalian cellsExploring the substrate range of wild-type aminoacyl-tRNA synthetases.Polyspecific pyrrolysyl-tRNA synthetases from directed evolutionNonsense and sense suppression abilities of original and derivative Methanosarcina mazei pyrrolysyl-tRNA synthetase-tRNA(Pyl) pairs in the Escherichia coli BL21(DE3) cell strainExpanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings.Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation.A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrumExpanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs.Cysteine Sulfoxidation Increases the Photostability of Red Fluorescent ProteinsGenetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader.Genetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells.Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutantGenetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label.Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation functionSmall-molecule control of protein function through Staudinger reduction.Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity.Proximity-enabled protein crosslinking through genetically encoding haloalkane unnatural amino acids.Genetic incorporation of seven ortho-substituted phenylalanine derivatives.Unnatural amino acid incorporation in E. coli: current and future applications in the design of therapeutic proteinsExpanded genetic code technologies for incorporating modified lysine at multiple sites.Genetically encoded photocrosslinkers for identifying and mapping protein-protein interactions in living cells.Genetically encoding new bioreactivity.Multiple site-selective insertions of noncanonical amino acids into sequence-repetitive polypeptides.Genetic incorporation of histidine derivatives using an engineered pyrrolysyl-tRNA synthetase.Genetically encoding an electrophilic amino acid for protein stapling and covalent binding to native receptors.Using Protein-Confined Proximity To Determine Chemical Reactivity.Genetic encoding of caged cysteine and caged homocysteine in bacterial and mammalian cells.Engineering the Genetic Code in Cells and Animals: Biological Considerations and Impacts.Site-specific incorporation of phosphotyrosine using an expanded genetic code.Pyrrolysine Amber Stop-Codon Suppression: Development and Applications.Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis.Genetically Encoding Fluorosulfate-l-tyrosine To React with Lysine, Histidine, and Tyrosine via SuFEx in Proteins in Vivo.Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue.
P2860
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P2860
Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids
description
2011 nî lūn-bûn
@nan
2011 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
2011 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
2011年の論文
@ja
2011年論文
@yue
2011年論文
@zh-hant
2011年論文
@zh-hk
2011年論文
@zh-mo
2011年論文
@zh-tw
2011年论文
@wuu
name
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@ast
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@en
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@nl
type
label
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@ast
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@en
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@nl
prefLabel
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@ast
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@en
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@nl
P2093
P2860
P3181
P356
P1433
P1476
Stereochemical Basis for Engin ...... vergent Non-native Amino Acids
@en
P2093
Jeffrey K Takimoto
Joseph P Noel
Nikki Dellas
P2860
P304
P3181
P356
10.1021/CB200057A
P50
P577
2011-07-15T00:00:00Z