Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
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EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule latticeStructural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complexCrystal structures of SCP2-thiolases of Trypanosomatidae, human pathogens causing widespread tropical diseases: the importance for catalysis of the cysteine of the unique HDCF loopYeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1Identification of the roles of conserved charged residues in the extracellular domain of an epithelial sodium channel (ENaC) subunit by alanine mutagenesisConserved charged residues at the surface and interface of epithelial sodium channel subunits--roles in cell surface expression and the sodium self-inhibition responseOne-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution CollectionsBiochemical and structural characterization of the interface mediating interaction between the influenza A virus non-structural protein-1 and a monoclonal antibodyCytoskeletal perturbation leads to platelet dysfunction and thrombocytopenia in variant forms of Glanzmann thrombastheniaHigh expression levels of the D686N Parkinson's disease mutation in VPS35 induces α-synuclein-dependent toxicity in yeastComputational and experimental approaches to reveal the effects of single nucleotide polymorphisms with respect to disease diagnosticsUracil DNA glycosylase BKRF3 contributes to Epstein-Barr virus DNA replication through physical interactions with proteins in viral DNA replication complex.Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli.Single residue mutation in active site of serine acetyltransferase isoform 3 from Entamoeba histolytica assists in partial regaining of feedback inhibition by cysteine.A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis.SOMA: a single oligonucleotide mutagenesis and cloning approachBGLF4 kinase modulates the structure and transport preference of the nuclear pore complex to facilitate nuclear import of Epstein-Barr virus lytic proteinsThe RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1.Exploring the protein stability landscape: Bacillus subtilis lipase A as a model for detergent tolerance.Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassemblyThe MazEF Toxin-Antitoxin System Alters the β-Lactam Susceptibility of Staphylococcus aureusFormation of a Trimeric Xpo1-Ran[GTP]-Ded1 Exportin Complex Modulates ATPase and Helicase Activities of Ded1The 3A protein from multiple picornaviruses utilizes the golgi adaptor protein ACBD3 to recruit PI4KIIIβ.Novel katG mutations causing isoniazid resistance in clinical M. tuberculosis isolates.Human and murine APOBEC3s restrict replication of koala retrovirus by different mechanisms.Active Hydrogen Bond Network (AHBN) and Applications for Improvement of Thermal Stability and pH-Sensitivity of Pullulanase from Bacillus naganoensisLoss of a Functionally and Structurally Distinct ld-Transpeptidase, LdtMt5, Compromises Cell Wall Integrity in Mycobacterium tuberculosis.Understanding renal nuclear protein accumulation: an in vitro approach to explain an in vivo phenomenon.Quantitative measurement of transcriptional inhibition and mutagenesis induced by site-specifically incorporated DNA lesions in vitro and in vivoThe inhibitory effect of ABA on floral transition is mediated by ABI5 in Arabidopsis.Mutational activation of the AmgRS two-component system in aminoglycoside-resistant Pseudomonas aeruginosa.Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coliMicrofluidic affinity and ChIP-seq analyses converge on a conserved FOXP2-binding motif in chimp and human, which enables the detection of evolutionarily novel targets.An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea.Friedelin Synthase from Maytenus ilicifolia: Leucine 482 Plays an Essential Role in the Production of the Most Rearranged Pentacyclic Triterpene.Protein engineering for metabolic engineering: current and next-generation tools.The nucleoid occlusion protein SlmA is a direct transcriptional activator of chitobiose utilization in Vibrio cholerae.MexY-promoted aminoglycoside resistance in Pseudomonas aeruginosa: involvement of a putative proximal binding pocket in aminoglycoside recognition.REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering.A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity.
P2860
Q27316013-B6B9DCC7-659A-41FF-915F-2DEED9971F11Q27677340-C64FC238-624B-42CE-8D0C-2122ECC57A00Q27685322-25FCADF1-81F8-48AC-B3FD-58DF11DD5EBBQ28115864-979E8BEC-6E0E-4152-85A0-1A505292592CQ28302470-44B99CC4-87A9-4B4A-9E2E-65A6437AC93BQ28308037-410EFD22-28E9-4390-BC2C-8DA81A431E4EQ28553645-E44C7269-CDBD-409D-82F2-84CFC93ABA45Q30392999-07754516-9E98-462D-835D-71F60D305D43Q33426579-65A53701-4E51-45DE-9ECB-D70D0D2A75A8Q33827945-A4361848-66D7-4CD9-82BD-98D3C4DD377CQ33907569-F9810E3C-558D-4155-885F-F1F9F503F1F6Q34059171-F73CBB1E-6A19-48E1-8643-514CB8B60F3FQ34095655-E907BDA0-B1C3-4F83-97C9-96E3DCA0128DQ34598288-B1A6D48B-FE03-40A9-8D2C-CEBB2450A903Q34622331-DAEB4175-DD1E-4AEF-BD1B-0456B1C8A384Q34764129-B259BDC3-1EDA-4AB0-A57B-E619EFB93514Q34991450-F3F310E9-162D-4E6B-917A-4842E7FAE87FQ35076146-2D91F053-C524-4E12-9377-84F5B3328DDCQ35577703-D36454BE-FA21-45E8-A2EB-F372B4CD7348Q35594571-A1EDDE5B-A167-4FBC-A0E9-0C65EF0F21E8Q35597691-3B1D16C9-E2B8-4B03-B435-517637192021Q35676923-0CD6460B-19AF-4377-B09A-A802FE590511Q35826912-C35B313B-20C0-4607-B6F8-FE9A573D64CBQ35909558-62993F65-D3A7-44EB-92E9-0EC6952F26E6Q35927461-C491327F-DF2B-4E03-85C5-334D4C7B8505Q36253808-F394287F-64D9-4B5C-B20B-B1E266A5BAA6Q36283392-A252F7C3-3589-4041-A7AF-2190F056159EQ36357444-E723D821-B011-49E3-843E-56C6A862F252Q36440706-BDA8B946-03E4-4F90-891E-E16C4F62EEA6Q36523826-EBEC6BB2-F0F9-49A5-BB5B-8E21B6C9A359Q36785893-68EE7DD6-7EE1-499F-A110-8C6552EA624AQ36790925-1A6A1DB7-EC1D-4B90-9110-84FB8FABBC0DQ36963289-193C0716-4CC8-467F-9F83-D6B02837B328Q37254448-553F7A17-BF0B-4A66-A902-E7D9F788016CQ37428315-27E66D78-3EE9-499E-BEC0-24FF0F9E735FQ38099295-319AA799-7D53-471B-AA70-54AE62AEC53FQ38287795-3A649BEA-4E89-4FFA-AA16-BE8F2150C9B0Q38873261-07366E95-88C1-4861-AB13-CF81856D4569Q40988187-79AC2623-4BEF-4367-BC92-B344D99BF1D2Q41244644-8134C5A1-8F03-4CA9-95C2-3C9DB3F02FFE
P2860
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
description
2009 nî lūn-bûn
@nan
2009 թուականին հրատարակուած գիտական յօդուած
@hyw
2009 թվականին հրատարակված գիտական հոդված
@hy
2009年の論文
@ja
2009年論文
@yue
2009年論文
@zh-hant
2009年論文
@zh-hk
2009年論文
@zh-mo
2009年論文
@zh-tw
2009年论文
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name
Simple and efficient site-dire ...... ein structure-function studies
@ast
Simple and efficient site-dire ...... ein structure-function studies
@en
Simple and efficient site-dire ...... ein structure-function studies
@en-gb
Simple and efficient site-dire ...... ein structure-function studies
@nl
type
label
Simple and efficient site-dire ...... ein structure-function studies
@ast
Simple and efficient site-dire ...... ein structure-function studies
@en
Simple and efficient site-dire ...... ein structure-function studies
@en-gb
Simple and efficient site-dire ...... ein structure-function studies
@nl
prefLabel
Simple and efficient site-dire ...... ein structure-function studies
@ast
Simple and efficient site-dire ...... ein structure-function studies
@en
Simple and efficient site-dire ...... ein structure-function studies
@en-gb
Simple and efficient site-dire ...... ein structure-function studies
@nl
P2860
P3181
P356
P1433
P1476
Simple and efficient site-dire ...... ein structure-function studies
@en
P2093
Oded Edelheit
P2860
P2888
P3181
P356
10.1186/1472-6750-9-61
P407
P577
2009-01-01T00:00:00Z
P5875
P6179
1016004458