Site-directed mutagenesis using double-stranded plasmid DNA templates.
about
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studiesMost efficient cocaine hydrolase designed by virtual screening of transition statesEnhancer scanning to locate regulatory regions in genomic lociLong-acting cocaine hydrolase for addiction therapyStructure and Biochemical Characterization of Protein Acetyltransferase from Sulfolobus solfataricusStructure of a Ternary Naa50p (NAT5/SAN) N-terminal Acetyltransferase Complex Reveals the Molecular Basis for Substrate-specific AcetylationDifferential Function of Lip Residues in the Mechanism and Biology of an Anthrax HemophoreMolecular basis for N-terminal acetylation by the heterodimeric NatA complexFunctional consequences of a novel variant of PCSK1The Relationship between Glycan Binding and Direct Membrane Interactions in Vibrio cholerae Cytolysin, a Channel-forming Toxin.Design of high-activity mutants of human butyrylcholinesterase against (-)-cocaine: structural and energetic factors affecting the catalytic efficiency.A novel allele of kaiA shortens the circadian period and strengthens interaction of oscillator components in the cyanobacterium Synechococcus elongatus PCC 7942.Orthologs of the class A4 heat shock transcription factor HsfA4a confer cadmium tolerance in wheat and rice.Biochemical analysis of MST1 kinase: elucidation of a C-terminal regulatory region.GroEL actively stimulates folding of the endogenous substrate protein PepQHeterologous overexpression and mutagenesis of the human bile salt export pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis).Role of helix B residues in interfacial activation of a bacterial phosphatidylinositol-specific phospholipase C.Identification of domains and amino acids essential to the collagen galactosyltransferase activity of GLT25D1.Characterization of P40, a cytadhesin of Mycoplasma agalactiae.Computational redesign of human butyrylcholinesterase for anticocaine medication.Structural variation in human apolipoprotein E3 and E4: secondary structure, tertiary structure, and size distribution.Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysisSDM-Assist software to design site-directed mutagenesis primers introducing "silent" restriction sitesGenetics of vitamin D 1alpha-hydroxylase deficiency in 17 familiesA mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis.Mycobacterium tuberculosis ClpP proteases are co-transcribed but exhibit different substrate specificities.Transient receptor potential melastatin 1 (TRPM1) is an ion-conducting plasma membrane channel inhibited by zinc ions.SOMA: a single oligonucleotide mutagenesis and cloning approachThe duplicated deacetylases Sir2 and Hst1 subfunctionalized by acquiring complementary inactivating mutations.Reversible unfolding of the severe acute respiratory syndrome coronavirus main protease in guanidinium chlorideA zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization.Aerobic Growth of Escherichia coli Is Reduced, and ATP Synthesis Is Selectively Inhibited when Five C-terminal Residues Are Deleted from the ϵ Subunit of ATP Synthase.Discovery of a specific inhibitor of human GLUT5 by virtual screening and in vitro transport evaluation.Inhibition of human GLUT1 and GLUT5 by plant carbohydrate products; insights into transport specificity.Modeling and Re-Engineering of Azotobacter vinelandii Alginate Lyase to Enhance Its Catalytic Efficiency for Accelerating Biofilm DegradationEvolution of new function through a single amino acid change in the yeast repressor Sum1pProtein kinase CK2 as an ectokinase: the role of the regulatory CK2beta subunit.Investigating the Role of Loop C Hydrophilic Residue 'T244' in the Binding Site of ρ1 GABAC Receptors via Site Mutation and Partial Agonism.Free-energy perturbation simulation on transition states and redesign of butyrylcholinesterase.Protein phosphatase 2A is regulated by protein kinase Cα (PKCα)-dependent phosphorylation of its targeting subunit B56α at Ser41.
P2860
Q21256641-BF4D2372-0111-4B26-A299-CCEDFEEC3C33Q24649976-D7D042B9-4FEE-483B-BEDA-5DAF78F0ED06Q27310315-95CAC405-2AAF-4D9A-AD9E-1FF099660004Q27318340-A43E7111-0A7A-4214-BBE5-E54333742C6EQ27655636-EF836593-7274-4E72-8C67-422CD4294B84Q27673397-3B50E339-9809-4849-ACA5-28FA94041702Q27678024-C9CF4961-12D3-4281-87EE-AB9F0572C4ACQ27679359-584624FE-BFF4-45F3-B434-48C1E65965DFQ28485317-D496000C-0889-4BFB-BE6A-F1D3243D94B4Q30379698-1AA60D0F-0C6A-4E79-B53F-60343E0E201BQ30394352-DC7875DD-2B93-4913-956D-C217DAD8BA78Q33435176-7B962F18-F87C-4931-844D-8AD1D1CC7102Q33520396-E32FB0FF-26BF-478B-9277-00D4ADBB960FQ33751017-C24A4AF7-79F6-45AE-9042-59826D51FCCFQ33871444-77075D56-7DE0-4776-9E39-299CAA8ACDF5Q33927067-41F17BE5-E846-4DE8-B85D-848EB59CF9C3Q34006081-0E9DE52A-FA95-4370-A914-FEDE6C239A32Q34117547-04D4479B-7A3B-4D17-952D-28F0AF1AEDDCQ34132900-B7124E15-EB0A-4779-8F1E-2A7FB5132F87Q34133353-A0EEF119-8C1B-4CF0-8781-08DAAF31D376Q34188538-AFC6240A-4668-46CB-9193-66FF0CB4C291Q34298714-DB8E2244-12D7-4D21-9C8B-889019F211DAQ34334823-FF59BB80-F30B-46A5-BF8D-11641D43649CQ34388188-97AF390F-C4C0-478C-AC1C-C2BA5A532508Q34622331-FFCF20EF-A6ED-4593-B451-07E9931DD0B6Q34656348-10121090-3801-45A4-9D43-8813C87FEF0FQ34752155-F14A5DB5-8DBC-4A51-A129-73677490F3D1Q34764129-4C34ECCF-51B7-49DE-A23B-40EBCBB910B2Q35139944-899A4751-52F2-417D-830A-94C467056D80Q35613695-06DDE95A-7B75-464A-A93D-C571B510B6D0Q35804661-FC6C3D63-0980-4798-9F71-3E9A47FC7270Q35978276-C4FF2A2E-24BD-4204-8C50-F0BB0D3730ABQ35988835-BC85FCAF-0253-4EB5-B06E-DD88EAC67BBFQ35996552-B87E3665-B311-4AF6-ACE8-AB6C39F468A1Q36037754-62656027-DF0C-4B7B-B7E8-542AF8CE656CQ36540275-3594AAED-8C24-4586-A797-89A63A8A6461Q36557945-1C58B066-7AF3-4779-AFC8-E46CD0D35455Q36951500-23ABFB7A-AEDD-473B-935A-4A82FC443766Q37279425-3607A90A-BDD4-4899-80CC-A7A9F8E2B597Q37428610-BF907BE8-FE69-4FE0-8A33-C04BAFB58EFC
P2860
Site-directed mutagenesis using double-stranded plasmid DNA templates.
description
1996 nî lūn-bûn
@nan
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
1996年论文
@zh
1996年论文
@zh-cn
name
Site-directed mutagenesis using double-stranded plasmid DNA templates.
@en
type
label
Site-directed mutagenesis using double-stranded plasmid DNA templates.
@en
prefLabel
Site-directed mutagenesis using double-stranded plasmid DNA templates.
@en
P2093
P356
P1476
Site-directed mutagenesis using double-stranded plasmid DNA templates.
@en
P2093
P356
10.1385/0-89603-332-5:31
P407
P577
1996-01-01T00:00:00Z