Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site
about
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesinA nanoscale force probe for gauging intermolecular interactionsThe origins of femtomolar protein-ligand binding: hydrogen-bond cooperativity and desolvation energetics in the biotin-(strept)avidin binding siteEvolved streptavidin mutants reveal key role of loop residue in high-affinity bindingStreptavidin and its biotin complex at atomic resolutionThe structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits.Structural consequences of cutting a binding loop: two circularly permuted variants of streptavidinStructure of Bradavidin – C-Terminal Residues Act as Intrinsic LigandsContamination from an affinity column: an encounter with a new villain in the world of membrane-protein crystallizationStructure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutantStructure-based design of a streptavidin mutant specific for an artificial biotin analogueTowards a rigorous network of protein-protein interactions of the model sulfate reducer Desulfovibrio vulgaris HildenboroughSubunit-specific inhibition of acid sensing ion channels by stomatin-like protein 1Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.Analysis of ER resident proteins in Saccharomyces cerevisiae: implementation of H/KDEL retrieval sequences.Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligandsSynthesis of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher chemistry.Inclusion of Strep-tag II in design of antigen receptors for T-cell immunotherapy.SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.A multienzyme complex channels substrates and electrons through acetyl-CoA and methane biosynthesis pathways in Methanosarcina.Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy.Structural characterization of core-bradavidin in complex with biotin.Assembly and use of high-density recombinant peptide chips for large-scale ligand screening is a practical alternative to synthetic peptide libraries.Directed evolution of streptavidin variants using in vitro compartmentalization.Nanomechanics of streptavidin hubs for molecular materialsExploiting features of adenovirus replication to support mammalian kinase productionSeveral affinity tags commonly used in chromatographic purification.Overview of Recent Progress in Protein-Expression Technologies for Small-Molecule Screening.Nanomechanical properties of protein-DNA layers with different oligonucleotide tethers.Transtactin: a universal transmembrane delivery system for Strep-tag II-fused cargos.The soluble NAD+-Reducing [NiFe]-hydrogenase from Ralstonia eutropha H16 consists of six subunits and can be specifically activated by NADPH.Single and multiple bonds in (strept)avidin-biotin interactions.Structure-based rational design of streptavidin mutants with pseudo-catalytic activity.Small-molecule inhibition of STOML3 oligomerization reverses pathological mechanical hypersensitivity.Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis.-Formulated Oligomers of Strep-Tagged Avian Influenza Haemagglutinin Produced in Plants Cause Neutralizing Immune Responses
P2860
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P2860
Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site
description
2002 nî lūn-bûn
@nan
2002 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
2002 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
2002年の論文
@ja
2002年論文
@yue
2002年論文
@zh-hant
2002年論文
@zh-hk
2002年論文
@zh-mo
2002年論文
@zh-tw
2002年论文
@wuu
name
Improved affinity of engineere ...... -like loop at the binding site
@ast
Improved affinity of engineere ...... -like loop at the binding site
@en
Improved affinity of engineere ...... -like loop at the binding site
@nl
type
label
Improved affinity of engineere ...... -like loop at the binding site
@ast
Improved affinity of engineere ...... -like loop at the binding site
@en
Improved affinity of engineere ...... -like loop at the binding site
@nl
prefLabel
Improved affinity of engineere ...... -like loop at the binding site
@ast
Improved affinity of engineere ...... -like loop at the binding site
@en
Improved affinity of engineere ...... -like loop at the binding site
@nl
P2860
P3181
P356
P1433
P1476
Improved affinity of engineere ...... -like loop at the binding site
@en
P2093
Ingo P Korndörfer
P2860
P304
P3181
P356
10.1110/PS.4150102
P50
P577
2002-04-01T00:00:00Z