Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components.
about
Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcriptsCwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centreFunctional organization of the Sm core in the crystal structure of human U1 snRNPThe organization and contribution of helicases to RNA splicingFunctional roles of DExD/H-box RNA helicases in Pre-mRNA splicingRegulation of DEAH/RHA helicases by G-patch proteinsRNA helicases in splicingSplicing fidelity: DEAD/H-box ATPases as molecular clocksNew insights into the spliceosome by single molecule fluorescence microscopyRNA helicase proteins as chaperones and remodelersPub1p C-Terminal RRM Domain Interacts with Tif4631p through a Conserved Region Neighbouring the Pab1p Binding SiteCrystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding proteinCooperative structure of the heterotrimeric pre-mRNA retention and splicing complexStructure of a yeast catalytic step I spliceosome at 3.4 Å resolutionCryo-EM structure of the spliceosome immediately after branchingMolecular architecture of the Saccharomyces cerevisiae activated spliceosomeQuality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1.The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome.Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosomeSplicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway.Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system.Single-Molecule Pull-Down FRET to Dissect the Mechanisms of Biomolecular MachinesSpliceosome Structure and FunctionRNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing.A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing.A bird's-eye view of post-translational modifications in the spliceosome and their roles in spliceosome dynamicsSupraspliceosomes at defined functional states portray the pre-assembled nature of the pre-mRNA processing machine in the cell nucleus.Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis.Rearrangements within human spliceosomes captured after exon ligation.Restraint of the G2/M transition by the SR/RRM family mRNA shuttling binding protein SNXAHRB1 in Aspergillus nidulans.RNA catalyses nuclear pre-mRNA splicingDEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps.Mechanisms and Regulation of Alternative Pre-mRNA SplicingCrystal structure, mutational analysis and RNA-dependent ATPase activity of the yeast DEAD-box pre-mRNA splicing factor Prp28.Structural requirement of Ntc77 for spliceosome activation and first catalytic step.GPKOW is essential for pre-mRNA splicing in vitro and suppresses splicing defect caused by dominant-negative DHX16 mutation in vivo.A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.
P2860
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P2860
Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components.
description
2009 nî lūn-bûn
@nan
2009 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2009 թվականի դեկտեմբերին հրատարակված գիտական հոդված
@hy
2009年の論文
@ja
2009年論文
@yue
2009年論文
@zh-hant
2009年論文
@zh-hk
2009年論文
@zh-mo
2009年論文
@zh-tw
2009年论文
@wuu
name
Reconstitution of both steps o ...... ified spliceosomal components.
@ast
Reconstitution of both steps o ...... ified spliceosomal components.
@en
Reconstitution of both steps o ...... ified spliceosomal components.
@nl
type
label
Reconstitution of both steps o ...... ified spliceosomal components.
@ast
Reconstitution of both steps o ...... ified spliceosomal components.
@en
Reconstitution of both steps o ...... ified spliceosomal components.
@nl
prefLabel
Reconstitution of both steps o ...... ified spliceosomal components.
@ast
Reconstitution of both steps o ...... ified spliceosomal components.
@en
Reconstitution of both steps o ...... ified spliceosomal components.
@nl
P2093
P2860
P3181
P356
P1476
Reconstitution of both steps o ...... ified spliceosomal components.
@en
P2093
Florian Platzmann
Henning Urlaub
Holger Stark
Jana Schmitzová
Patrizia Fabrizio
Peter Odenwälder
Ralf Ficner
P2860
P2888
P304
P3181
P356
10.1038/NSMB.1729
P407
P577
2009-12-01T00:00:00Z
P5875
P6179
1030588247