Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: high-yield expression and purification of human P-glycoprotein.
about
The remarkable transport mechanism of P-glycoprotein: a multidrug transporter.Heterologous expression and purification systems for structural proteomics of mammalian membrane proteinsA novel electron paramagnetic resonance approach to determine the mechanism of drug transport by P-glycoprotein.Molecular architecture and subunit organization of TRPA1 ion channel revealed by electron microscopyTuning microbial hosts for membrane protein production.Expression, purification and structural properties of ABC transporter ABCA4 and its individual domains.Heterologous overexpression and mutagenesis of the human bile salt export pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis).Epitope tagging of the yeast K(+) carrier Trk2p demonstrates folding that is consistent with a channel-like structure.Quantitative modeling of chloride conductance in yeast TRK potassium transportersA synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis.Anion currents in yeast K+ transporters (TRK) characterize a structural homologue of ligand-gated ion channels.Overexpression of membrane proteins from higher eukaryotes in yeasts.High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.Endoplasmic Reticulum Stress and Unfolded Protein Response Pathways: Potential for Treating Age-related Retinal DegenerationCD44 promotes multi-drug resistance by protecting P-glycoprotein from FBXO21-mediated ubiquitinationStructure of TRPV1 channel revealed by electron cryomicroscopyUnfolding the Therapeutic Potential of Chemical Chaperones for Age-related Macular DegenerationP-glycoprotein is fully active after multiple tryptophan substitutionsFunctional and structural studies of TRP channels heterologously expressed in budding yeast.Allosteric modulation of the substrate specificity of acyl-CoA wax alcohol acyltransferase 2.Integral Membrane Protein Expression in Saccharomyces cerevisiae.Coordination of K+ transporters in neurospora: TRK1 is scarce and constitutive, while HAK1 is abundant and highly regulated.Nitrogen catabolite repressible GAP1 promoter, a new tool for efficient recombinant protein production in S. cerevisiae.New high-throughput screening assay to reveal similarities and differences in inhibitory sensitivities of multidrug ATP-binding cassette transporters.Transport of leukotriene C4 by a cysteine-less multidrug resistance protein 1 (MRP1).Transition state analysis of the coupling of drug transport to ATP hydrolysis by P-glycoprotein.N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase from Entamoeba histolytica: metal alters catalytic rates but not substrate affinity.An improved method for the cost-effective expression and purification of large quantities of KcsA.Impact of LCA-Associated E14L LRAT Mutation on Protein Stability and Retinoid Homeostasis.Cysteines 431 and 1074 are responsible for inhibitory disulfide cross-linking between the two nucleotide-binding sites in human P-glycoprotein.P-glycoprotein catalytic mechanism: studies of the ADP-vanadate inhibited state.Specific structural requirements for the inhibitory effect of thapsigargin on the Ca2+ ATPase SERCA.Recombinant synthesis of human ABCG2 expressed in the yeast Saccharomyces cerevisiae: an experimental methodological study.Saccharomyces cerevisiae Gpi2, an accessory subunit of the enzyme catalyzing the first step of glycosylphosphatidylinositol (GPI) anchor biosynthesis, selectively complements some of the functions of its homolog in Candida albicans.Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes.A cost-effective protocol for the over-expression and purification of fully-functional and more stable Erwinia chrysanthemi ligand-gated ion channel.Membrane Protein Production in the Yeast, S. cerevisiae.Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels
P2860
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P2860
Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: high-yield expression and purification of human P-glycoprotein.
description
2000 nî lūn-bûn
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2000 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
2000 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
2000年の論文
@ja
2000年論文
@yue
2000年論文
@zh-hant
2000年論文
@zh-hk
2000年論文
@zh-mo
2000年論文
@zh-tw
2000年论文
@wuu
name
Use of chemical chaperones in ...... ation of human P-glycoprotein.
@ast
Use of chemical chaperones in ...... ation of human P-glycoprotein.
@en
type
label
Use of chemical chaperones in ...... ation of human P-glycoprotein.
@ast
Use of chemical chaperones in ...... ation of human P-glycoprotein.
@en
prefLabel
Use of chemical chaperones in ...... ation of human P-glycoprotein.
@ast
Use of chemical chaperones in ...... ation of human P-glycoprotein.
@en
P2093
P356
P1476
Use of chemical chaperones in ...... ation of human P-glycoprotein.
@en
P2093
P356
10.1006/ABBI.2000.1712
P407
P577
2000-04-01T00:00:00Z