Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.
about
Tuning the dials of Synthetic BiologyExploring protein fitness landscapes by directed evolutionDiscovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteinsTriplet nucleotide removal at random positions in a target gene: the tolerance of TEM-1 beta-lactamase to an amino acid deletion.A computer program for the estimation of protein and nucleic acid sequence diversity in random point mutagenesis libraries.Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolutionRAISE: a simple and novel method of generating random insertion and deletion mutationsEvolution of a designed retro-aldolase leads to complete active site remodelingDirected evolution: tailoring biocatalysts for industrial applicationsOne-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution CollectionsRecombining low homology, functionally rich regions of bacterial subtilisins by combinatorial fragment exchangeInvestigating protein structural plasticity by surveying the consequence of an amino acid deletion from TEM-1 beta-lactamase.Computationally mapping sequence space to understand evolutionary protein engineering.Directed evolution of a histone acetyltransferase--enhancing thermostability, whilst maintaining catalytic activity and substrate specificity.Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution.Directed evolution of a bacterial efflux pump: adaptation of the E. coli TolC exit duct to the Pseudomonas MexAB translocase.Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries.Improved synthesis of trinucleotide phosphoramidites and generation of randomized oligonucleotide libraries.Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries.Revealing biases inherent in recombination protocols.Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx).Predicting protein evolution in vitro by phage escape technology.TrimerDimer: an oligonucleotide-based saturation mutagenesis approach that removes redundant and stop codonsConstruction and maintenance of randomized retroviral expression libraries for transmembrane protein engineering.Genomes by design.An experimentally determined evolutionary model dramatically improves phylogenetic fit.Microarray generation of thousand-member oligonucleotide libraries.Enhancing the Clinical Potential of AAV Vectors by Capsid Engineering to Evade Pre-Existing Immunity.Synthetic Biology: A Bridge between Artificial and Natural CellsLessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.Directed Evolution of Enzymes for Industrial Biocatalysis.Exploring the potential of xenobiotic-metabolising enzymes as biocatalysts: evolving designer catalysts from polyfunctional cytochrome P450 enzymes.Engineering sortase A by screening a second-generation library using phage display.The outlook for protein engineering in crop improvement.Directing the evolution of Rubisco and Rubisco activase: first impressions of a new tool for photosynthesis research.Nucleic acid and peptide aptamers: fundamentals and bioanalytical aspects.Approaches to chemical synthetic biology.Enzymes to die for: exploiting nucleotide metabolizing enzymes for cancer gene therapy.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Designer protein delivery: From natural to engineered affinity-controlled release systems.
P2860
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P2860
Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.
description
2004 nî lūn-bûn
@nan
2004 թուականի Փետրուարին հրատարակուած գիտական յօդուած
@hyw
2004 թվականի փետրվարին հրատարակված գիտական հոդված
@hy
2004年の論文
@ja
2004年論文
@yue
2004年論文
@zh-hant
2004年論文
@zh-hk
2004年論文
@zh-mo
2004年論文
@zh-tw
2004年论文
@wuu
name
Chemical and biochemical strat ...... ethods for directed evolution.
@ast
Chemical and biochemical strat ...... ethods for directed evolution.
@en
type
label
Chemical and biochemical strat ...... ethods for directed evolution.
@ast
Chemical and biochemical strat ...... ethods for directed evolution.
@en
prefLabel
Chemical and biochemical strat ...... ethods for directed evolution.
@ast
Chemical and biochemical strat ...... ethods for directed evolution.
@en
P2860
P356
P1476
Chemical and biochemical strat ...... ethods for directed evolution.
@en
P2860
P304
P356
10.1093/NAR/GKH315
P407
P577
2004-02-27T00:00:00Z