In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates
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Exploring protein fitness landscapes by directed evolutionAnticipatory evolution and DNA shufflingEvolution of genetic potential.Peptidoglycan remodeling by the coordinated action of multispecific enzymesFollowing evolutionary paths to protein-protein interactions with high affinity and selectivityStructural Basis of a Rationally Rewired Protein-Protein Interface Critical to Bacterial SignalingThe 'evolvability' of promiscuous protein functionsHIV-1 proviral DNA excision using an evolved recombinaseCatalytic and binding poly-reactivities shared by two unrelated proteins: The potential role of promiscuity in enzyme evolutionA thermostable β-glucuronidase obtained by directed evolution as a reporter gene in transgenic plantsEvolutionary history of a specialized p450 propane monooxygenaseAdvances in directed molecular evolution of reporter genes.Guiding bacteria with small molecules and RNA.Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.The quest for molecular quasi-species in ligand-activity space and its application to directed enzyme evolution.Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis.Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop.Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.Laboratory-directed protein evolutionDiversification and specialization of HIV protease function during in vitro evolution.The evolution of catalytic efficiency and substrate promiscuity in human theta class 1-1 glutathione transferase.Emergence of novel functions in transcriptional regulators by regression to stem protein types.Non-contact positions impose site selectivity on Cre recombinase.Applying neutral drift to the directed molecular evolution of a β-glucuronidase into a β-galactosidase: Two different evolutionary pathways lead to the same variant.Designed divergent evolution of enzyme function.Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferaseThe independent prokaryotic origins of eukaryotic fructose-1, 6-bisphosphatase and sedoheptulose-1, 7-bisphosphatase and the implications of their origins for the evolution of eukaryotic Calvin cycle.Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease.Structure and Inhibition of Microbiome β-Glucuronidases Essential to the Alleviation of Cancer Drug ToxicityModifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution.OptZyme: computational enzyme redesign using transition state analoguesSubstrate scope and selectivity in offspring to an enzyme subjected to directed evolution.Panoramic view of a superfamily of phosphatases through substrate profilingImproved detection of Escherichia coli and coliform bacteria by multiplex PCRToward reducing immunogenicity of enzyme replacement therapy: altering the specificity of human β-glucuronidase to compensate for α-iduronidase deficiency.Directed evolution of highly selective proteases by using a novel FACS-based screen that capitalizes on the p53 regulator MDM2Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native ActivityControlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion.Evolution of a G protein-coupled receptor response by mutations in regulatory network interactions.Substrate ambiguous enzymes within the Escherichia coli proteome offer different evolutionary solutions to the same problem.
P2860
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P2860
In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates
description
2001 nî lūn-bûn
@nan
2001 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի հունվարին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@ast
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@en
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@nl
type
label
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@ast
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@en
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@nl
prefLabel
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@ast
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@en
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@nl
P3181
P356
P1476
In vitro evolution of beta-glu ...... ugh non-specific intermediates
@en
P2093
A D Ellington
I Matsumura
P3181
P356
10.1006/JMBI.2000.4259
P407
P577
2001-01-12T00:00:00Z