Yeast surface display for directed evolution of protein expression, affinity, and stability.
about
Mimotopes for alloreactive and conventional T cells in a peptide-MHC display libraryApplications of Yeast Surface Display for Protein EngineeringIdentification of Posttranslational Modification-Dependent Protein Interactions Using Yeast Surface Displayed Human Proteome LibrariesExploring the Capacity of Minimalist Protein Interfaces: Interface Energetics and Affinity Maturation to Picomolar KD of a Single-domain Antibody with a Flat ParatopeStructure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule FamilyToward chaperone-assisted crystallography: Protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffoldDeterminants of BH3 Binding Specificity for Mcl-1 versus Bcl-xLTeaching an Old Scaffold New Tricks: Monobodies Constructed Using Alternative Surfaces of the FN3 ScaffoldExpanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence spaceA Variable Light Domain Fluorogen Activating Protein Homodimerizes To Activate Dimethylindole RedYeast mating for combinatorial Fab library generation and surface displayHumanized antibody neutralizing 2009 pandemic H1N1 virus.Computationally mapping sequence space to understand evolutionary protein engineering.Tailor-made biocatalysts: combining thermodynamics, organic synthesis, molecular biology, biochemistry and microbiology for the design of enzyme selections.Yeast Surface-Displayed H5N1 Avian Influenza Vaccines.Isolation of a high affinity neutralizing monoclonal antibody against 2009 pandemic H1N1 virus that binds at the 'Sa' antigenic site.Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.Flow cytometry-based methods for assessing soluble scFv activities and detecting antigens in solution.Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries.Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed libraries.Fluorescence-activated cell sorting of specific affibody-displaying staphylococci.A random peptide library fused to CCR5 for selection of mimetopes expressed on the mammalian cell surface via retroviral vectors.An scFv intrabody against the nonamyloid component of alpha-synuclein reduces intracellular aggregation and toxicityA decade of yeast surface display technology: where are we now?High-throughput analysis of the protein sequence-stability landscape using a quantitative yeast surface two-hybrid system and fragment reconstitutionLibraries against libraries for combinatorial selection of replicating antigen-antibody pairsDetecting morphologically distinct oligomeric forms of alpha-synuclein.The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins.Engineered cystine knot peptides that bind alphavbeta3, alphavbeta5, and alpha5beta1 integrins with low-nanomolar affinity.Accelerating phage-display library selection by reversible and site-specific biotinylation.A versatile selection system for folding competent proteins using genetic complementation in a eukaryotic host.Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody.Development of a novel mammalian cell surface antibody display platformUse of self-actuating and self-sensing cantilevers for imaging biological samples in fluidAntibody buffering of a ligand in vivoDevelopment of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenasesHuman monoclonal antibody fragments binding to insulin-like growth factors I and II with picomolar affinity.Engineering hepatocyte growth factor fragments with high stability and activity as Met receptor agonists and antagonists.Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast.
P2860
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P2860
Yeast surface display for directed evolution of protein expression, affinity, and stability.
description
2000 nî lūn-bûn
@nan
2000 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
2000 թվականի հունվարին հրատարակված գիտական հոդված
@hy
2000年の論文
@ja
2000年論文
@yue
2000年論文
@zh-hant
2000年論文
@zh-hk
2000年論文
@zh-mo
2000年論文
@zh-tw
2000年论文
@wuu
name
Yeast surface display for dire ...... sion, affinity, and stability.
@ast
Yeast surface display for dire ...... sion, affinity, and stability.
@en
type
label
Yeast surface display for dire ...... sion, affinity, and stability.
@ast
Yeast surface display for dire ...... sion, affinity, and stability.
@en
prefLabel
Yeast surface display for dire ...... sion, affinity, and stability.
@ast
Yeast surface display for dire ...... sion, affinity, and stability.
@en
P1476
Yeast surface display for dire ...... sion, affinity, and stability.
@en
P2093
K D Wittrup
P304
P356
10.1016/S0076-6879(00)28410-3
P407
P577
2000-01-01T00:00:00Z