Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).
about
De novo proteins from designed combinatorial librariesIntrabody and Parkinson's diseaseComputational design of ligand-binding proteins with high affinity and selectivityDirected evolution: tailoring biocatalysts for industrial applicationsDirected evolution of a G protein-coupled receptor for expression, stability, and binding selectivityDesired alteration of protein affinities: competitive selection of protein variants using yeast signal transduction machineryStrategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes of transport across the inner membrane of bacteria.Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.Genetic analysis of the twin arginine translocator secretion pathway in bacteria.Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries.Target validation and drug discovery using genomic and protein-protein interaction technologies.Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway.Display technologies: application for the discovery of drug and gene delivery agents.A scFv antibody mutant isolated in a genetic screen for improved export via the twin arginine transporter pathway exhibits faster folding.Design of synthetic antibody libraries.Engineering and rapid selection of a low-affinity glucose/galactose-binding protein for a glucose biosensorIsolation of anti-toxin single domain antibodies from a semi-synthetic spiny dogfish shark display library.E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display.Discovery of amyloid-beta aggregation inhibitors using an engineered assay for intracellular protein folding and solubility.Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodiesIsolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexesPassive protection against anthrax by using a high-affinity antitoxin antibody fragment lacking an Fc regionMulti-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli.Folding quality control in the export of proteins by the bacterial twin-arginine translocation pathway.Strain engineering for improved expression of recombinant proteins in bacteriaA novel platform for antibody library selection in mammalian cells based on a growth signalobody.Rapid isolation of antibody from a synthetic human antibody library by repeated fluorescence-activated cell sorting (FACS)Domain structure of growth signalobodies critically affects the outcome of antibody library selection.Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display systemTranspo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries.Enhanced secretion of recombinant proteins via signal recognition particle (SRP)-dependent secretion pathway by deletion of rrsE in Escherichia coli.Engineered bacterial outer membrane vesicles with enhanced functionalityEngineering G protein-coupled receptor expression in bacteriaImmunoglobulin domains in Escherichia coli and other enterobacteria: from pathogenesis to applications in antibody technologies.A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution.Conserved amplification of chemotactic responses through chemoreceptor interactionsEvaluation of staphylococcal cell surface display and flow cytometry for postselectional characterization of affinity proteins in combinatorial protein engineering applications.Affinity maturation of an anti-V antigen IgG expressed in situ through adenovirus gene delivery confers enhanced protection against Yersinia pestis challenge.Exploration of twin-arginine translocation for expression and purification of correctly folded proteins in Escherichia coli.
P2860
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P2860
Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).
description
2001 nî lūn-bûn
@nan
2001 թուականի Յունիսին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի հունիսին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
Isolation of high-affinity lig ...... h cytometric screening (PECS).
@ast
Isolation of high-affinity lig ...... h cytometric screening (PECS).
@en
type
label
Isolation of high-affinity lig ...... h cytometric screening (PECS).
@ast
Isolation of high-affinity lig ...... h cytometric screening (PECS).
@en
prefLabel
Isolation of high-affinity lig ...... h cytometric screening (PECS).
@ast
Isolation of high-affinity lig ...... h cytometric screening (PECS).
@en
P2093
P2860
P356
P1433
P1476
Isolation of high-affinity lig ...... th cytometric screening (PECS)
@en
P2093
P2860
P2888
P304
P356
10.1038/89281
P577
2001-06-01T00:00:00Z