Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates.
about
Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design.Revisiting the lipase from Pseudomonas aeruginosa: directed evolution of substrate acceptance and enantioselectivity using iterative saturation mutagenesis.Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli.Enhancing the efficiency of directed evolution in focused enzyme libraries by the adaptive substituent reordering algorithm.An improved dual-tube megaprimer approach for multi-site saturation mutagenesis.Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library construction.A screening protocol for identification of functional mutants of RNA editing adenosine deaminases.Directed evolution by using iterative saturation mutagenesis based on multiresidue sites.A practical comparison of ligation-independent cloning techniquesBiomathematical description of synthetic peptide libraries.Economical analysis of saturation mutagenesis experiments.Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, from Aspergillus usamii by iterative saturation mutagenesis.The Regulatory Domain of Squalene Monooxygenase Contains a Re-entrant Loop and Senses Cholesterol via a Conformational Change.P450(BM3) (CYP102A1): connecting the dots.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.A quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues in HjLPMO9A regioselectivity.Bioinformatics for the synthetic biology of natural products: integrating across the Design-Build-Test cycle.Determining the Topology of Membrane-Bound Proteins Using PEGylation.Directed evolution of phenylacetone monooxygenase as an active catalyst for the Baeyer-Villiger conversion of cyclohexanone to caprolactone.A key regulator of cholesterol homoeostasis, SREBP-2, can be targeted in prostate cancer cells with natural products.Achieving regio- and enantioselectivity of P450-catalyzed oxidative CH activation of small functionalized molecules by structure-guided directed evolution.Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing.Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes.Assessing the correlation between mutant rhodopsin stability and the severity of retinitis pigmentosaCross-talk between the androgen receptor and the liver X receptor: implications for cholesterol homeostasis.Engineering of Thermomyces lanuginosus lipase Lip: creation of novel biocatalyst for efficient biosynthesis of chiral intermediate of Pregabalin.The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-phosphate phosphorylase as a novel specificity.Characterization and mutational analysis of the UDP-Glc(NAc) 4-epimerase from Marinithermus hydrothermalis.A conserved degron containing an amphipathic helix regulates the cholesterol-mediated turnover of human squalene monooxygenase, a rate-limiting enzyme in cholesterol synthesis.Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism.Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues.Engineering the carbohydrate-binding site of Epa1p from Candida glabrata: generation of adhesin mutants with different carbohydrate specificity.Disulfide bridges as essential elements for the thermostability of lytic polysaccharide monooxygenase LPMO10C from Streptomyces coelicolor.Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy.
P2860
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P2860
Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates.
description
2008 nî lūn-bûn
@nan
2008 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած
@hyw
2008 թվականի սեպտեմբերին հրատարակված գիտական հոդված
@hy
2008年の論文
@ja
2008年論文
@yue
2008年論文
@zh-hant
2008年論文
@zh-hk
2008年論文
@zh-mo
2008年論文
@zh-tw
2008年论文
@wuu
name
Improved PCR method for the cr ...... ifficult-to-amplify templates.
@ast
Improved PCR method for the cr ...... ifficult-to-amplify templates.
@en
type
label
Improved PCR method for the cr ...... ifficult-to-amplify templates.
@ast
Improved PCR method for the cr ...... ifficult-to-amplify templates.
@en
prefLabel
Improved PCR method for the cr ...... ifficult-to-amplify templates.
@ast
Improved PCR method for the cr ...... ifficult-to-amplify templates.
@en
P2093
P1476
Improved PCR method for the cr ...... ifficult-to-amplify templates.
@en
P2093
Andreas Taglieber
Daniel Kahakeaw
Felipe E Zilly
Horst Höbenreich
J Daniel Carballeira
Joaquin Sanchis
John Podtetenieff
Jullien Drone
Jérôme J-P Peyralans
Layla Fernández
P2888
P304
P356
10.1007/S00253-008-1678-9
P407
P577
2008-09-27T00:00:00Z
P5875
P6179
1046650831