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Multiplexed transposon-mediated stable gene transfer in human cells

Multiplexed transposon-mediated stable gene transfer in human cells

http://www.wikidata.org/entity/Q33667400

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Propranolol blocks cardiac and neuronal voltage-gated sodium channels Packaging HIV- or FIV-based lentivector expression constructs and transduction of VSV-G pseudotyped viral particles Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins Somatic genetics empowers the mouse for modeling and interrogating developmental and disease processes.KCNE4 juxtamembrane region is required for interaction with calmodulin and for functional suppression of KCNQ1.CHO cell lines generated by PiggyBac transposition.Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells Combinatorial proteomic analysis of intercellular signaling applied to the CD28 T-cell costimulatory receptor.PiggyBac mediated multiplex gene transfer in mouse embryonic stem cell PiggyBac-mediated cancer immunotherapy using EBV-specific cytotoxic T-cells expressing HER2-specific chimeric antigen receptor.Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model.Hyperactive piggyBac gene transfer in human cells and in vivo.PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells.piggyBac-ing models and new therapeutic strategies.Nontruncating SCN1A mutations associated with severe myoclonic epilepsy of infancy impair cell surface expression.Mechanism of sodium channel NaV1.9 potentiation by G-protein signaling Simple piggyBac transposon-based mammalian cell expression system for inducible protein production.Modular assembly of transposon integratable multigene vectors using RecWay assembly.Gene therapy strategies for cardiac electrical dysfunction.The expanding universe of transposon technologies for gene and cell engineering.Size matters: versatile use of PiggyBac transposons as a genetic manipulation tool.Manipulating piggyBac transposon chromosomal integration site selection in human cells Multiplexed gene transfer to a human T-cell line by combining Sleeping Beauty transposon system with methotrexate selection.A transposon-mediated system for flexible control of transgene expression in stem and progenitor-derived lineages.Minimal piggyBac vectors for chromatin integration.The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability.Sodium channel NaV1.9 mutations associated with insensitivity to pain dampen neuronal excitability.Evaluation of long-term transgene expression in piggyBac-modified human T lymphocytes.Vector modifications to eliminate transposase expression following piggyBac-mediated transgenesis piggyBac transposon system modification of primary human T cells.Multigene expression in stable CHO cell pools generated with the piggyBac transposon system.A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells.Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines.Renaissance of ion channel research and drug discovery by patch clamp automation.Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo.

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P2860

Multiplexed transposon-mediated stable gene transfer in human cells

http://www.wikidata.org/entity/Q33667400

description

2010 nî lūn-bûn

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2010 թուականի Յունուարին հրատարակուած գիտական յօդուած

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2010 թվականի հունվարին հրատարակված գիտական հոդված

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2010年の論文

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2010年論文

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2010年論文

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2010年論文

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2010年論文

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2010年論文

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2010年论文

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name

Multiplexed transposon-mediated stable gene transfer in human cells

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Multiplexed transposon-mediated stable gene transfer in human cells

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type

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Multiplexed transposon-mediated stable gene transfer in human cells

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Multiplexed transposon-mediated stable gene transfer in human cells

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prefLabel

Multiplexed transposon-mediated stable gene transfer in human cells

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Multiplexed transposon-mediated stable gene transfer in human cells

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P1476

Multiplexed transposon-mediated stable gene transfer in human cells

@en

P2093

Alfred L George

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Aparna Kaja

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Kristopher M Kahlig

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Matthew H Wilson

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Melissa A Daniels

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Sai K Saridey

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P2860

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Induced pluripotent stem cell lines derived from human somatic cells

Induction of pluripotent stem cells from adult human fibroblasts by defined factors

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells

piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells

Design and implementation of cell-based assays to model human disease.

A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells.

PiggyBac transposon-mediated gene transfer in human cells.

Chromosomal transposition of PiggyBac in mouse embryonic stem cells.

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20080581

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