Assembly and overexpression of membrane proteins in Escherichia coli.
about
A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation.High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1Crystallization of membrane proteins by vapor diffusion.Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coliPredominant membrane localization is an essential feature of the bacterial signal recognition particle receptor.Engineering membrane protein overproduction in Escherichia coli.Tuning microbial hosts for membrane protein production.Dynamic interaction of the sec translocon with the chaperone PpiDCo-translational association of cell-free expressed membrane proteins with supplied lipid bilayers.Matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosisTat peptide-mediated soluble expression of the membrane protein LSECtin-CRD in Escherichia coliMechanisms of integral membrane protein insertion and foldingOverexpression of membrane proteins from higher eukaryotes in yeasts.Physiological response to membrane protein overexpression in E. coli.Promiscuous targeting of polytopic membrane proteins to SecYEG or YidC by the Escherichia coli signal recognition particle.A semisynthetic Eph receptor tyrosine kinase provides insight into ligand-induced kinase activationRegulation by a chaperone improves substrate selectivity during cotranslational protein targetingEnhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screenA scalable, GFP-based pipeline for membrane protein overexpression screening and purification.Quality control of overexpressed membrane proteins.Membrane protein structural biology--how far can the bugs take us?Tuning Escherichia coli for membrane protein overexpressionFunctional expression and characterisation of membrane transport proteins.The Sec translocon mediated protein transport in prokaryotes and eukaryotes.Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli.Overexpression, purification and validation of antigenic Salmonella enterica serovar Typhi proteins identified from LC-MS/MS.Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.An Escherichia coli strain for expression of the connexin45 carboxyl terminus attached to the 4th transmembrane domain.Escherichia coli SRP, its protein subunit Ffh, and the Ffh M domain are able to selectively limit membrane protein expression when overexpressed.Studying of Membrane Localization of Recombinant Potassium Channels in E.coli.Bacterial expression of a eukaryotic membrane protein in fusion to various Mistic orthologsHigh-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG.Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions.Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.Targeting and translocation of two lipoproteins in Escherichia coli via the SRP/Sec/YidC pathway.Consequences of membrane protein overexpression in Escherichia coli.Reconstitution, spectroscopy, and redox properties of the photosynthetic recombinant cytochrome b(559) from higher plants.Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2.The Bacterial SRP Receptor, SecA and the Ribosome Use Overlapping Binding Sites on the SecY Translocon
P2860
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P2860
Assembly and overexpression of membrane proteins in Escherichia coli.
description
2003 nî lūn-bûn
@nan
2003 թուականի Փետրուարին հրատարակուած գիտական յօդուած
@hyw
2003 թվականի փետրվարին հրատարակված գիտական հոդված
@hy
2003年の論文
@ja
2003年論文
@yue
2003年論文
@zh-hant
2003年論文
@zh-hk
2003年論文
@zh-mo
2003年論文
@zh-tw
2003年论文
@wuu
name
Assembly and overexpression of membrane proteins in Escherichia coli.
@ast
Assembly and overexpression of membrane proteins in Escherichia coli.
@en
type
label
Assembly and overexpression of membrane proteins in Escherichia coli.
@ast
Assembly and overexpression of membrane proteins in Escherichia coli.
@en
prefLabel
Assembly and overexpression of membrane proteins in Escherichia coli.
@ast
Assembly and overexpression of membrane proteins in Escherichia coli.
@en
P2093
P1476
Assembly and overexpression of membrane proteins in Escherichia coli.
@en
P2093
Jan-Willem L de Gier
Linda Fröderberg
Louise Baars
P356
10.1016/S0005-2736(02)00707-1
P407
P50
P577
2003-02-01T00:00:00Z