A scalable, GFP-based pipeline for membrane protein overexpression screening and purification.
about
Membrane protein structure determination - the next generationThe Crystal Structure of Six-transmembrane Epithelial Antigen of the Prostate 4 (Steap4), a Ferri/Cuprireductase, Suggests a Novel Interdomain Flavin-binding SiteHigh-throughput recombinant protein expression in Escherichia coli: current status and future perspectivesMembrane protein production in Escherichia coli cell-free lysatesNanoscale Synaptic Membrane Mimetic Allows Unbiased High Throughput Screen That Targets Binding Sites for Alzheimer's-Associated Aβ Oligomers.Functional reconstitution of Staphylococcus aureus truncated AgrC histidine kinase in a model membrane system.Structural genomics target selection for the New York consortium on membrane protein structure.Overcoming barriers to membrane protein structure determination.Engineering membrane protein overproduction in Escherichia coli.Tuning microbial hosts for membrane protein production.Recombinant expression screening of P. aeruginosa bacterial inner membrane proteinsEnhanced expression of codon optimized Mycobacterium avium subsp. paratuberculosis antigens in Lactobacillus salivarius.Fluorescent probe for high-throughput screening of membrane protein expressionOvercoming the challenges of membrane protein crystallography.High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.Quality control of overexpressed membrane proteins.Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes.Efficient production of membrane-integrated and detergent-soluble G protein-coupled receptors in Escherichia coli.Tuning Escherichia coli for membrane protein overexpressionA Novel and Fast Purification Method for Nucleoside Transporters.A Link between Integral Membrane Protein Expression and Simulated Integration EfficiencyGreen fluorescent protein (GFP)-based overexpression screening and characterization of AgrC, a Receptor protein of quorum sensing in Staphylococcus aureusA green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organismsRevolutionizing membrane protein overexpression in bacteria.GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins.A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution.Expression, Solubilization, and Purification of Bacterial Membrane Proteins.De-bugging and maximizing plant cytochrome P450 production in Escherichia coli with C-terminal GFP fusions.A Two-component NADPH Oxidase (NOX)-like System in Bacteria Is Involved in the Electron Transfer Chain to the Methionine Sulfoxide Reductase MsrP.Rapid expression screening of eukaryotic membrane proteins in Pichia pastoris.Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini.Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1.Consequences of the overexpression of a eukaryotic membrane protein, the human KDEL receptor, in Escherichia coli.Mining mammalian genomes for folding competent proteins using Tat-dependent genetic selection in Escherichia coli.General flexible nature of the cytosolic regions of fungal transient receptor potential (TRP) channels, revealed by expression screening using GFP-fusion techniques.Method to increase the yield of eukaryotic membrane protein expression in Saccharomyces cerevisiae for structural and functional studies.Combinatorial method for overexpression of membrane proteins in Escherichia coli.Effect of detergents on the thermal behavior of elastin-like polypeptides.A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies
P2860
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P2860
A scalable, GFP-based pipeline for membrane protein overexpression screening and purification.
description
2005 nî lūn-bûn
@nan
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
2005年论文
@zh
2005年论文
@zh-cn
name
A scalable, GFP-based pipeline ...... on screening and purification.
@ast
A scalable, GFP-based pipeline ...... on screening and purification.
@en
type
label
A scalable, GFP-based pipeline ...... on screening and purification.
@ast
A scalable, GFP-based pipeline ...... on screening and purification.
@en
prefLabel
A scalable, GFP-based pipeline ...... on screening and purification.
@ast
A scalable, GFP-based pipeline ...... on screening and purification.
@en
P2093
P2860
P50
P356
P1433
P1476
A scalable, GFP-based pipeline ...... on screening and purification.
@en
P2093
Daniel O Daley
Dirk-Jan Slotboom
Giulia Friso
Jan-Willem De Gier
Klaas-Jan Van Wijk
Mirjam Lerch
Nadja M Meindl-Beinker
Torsten Reda
Wietske Lambert
P2860
P304
P356
10.1110/PS.051466205
P577
2005-06-29T00:00:00Z