Expression and partial purification of enzymatically active recombinant Ty1 integrase in Saccharomyces cerevisiae
about
Retrotransposons. An RNA polymerase III subunit determines sites of retrotransposon integration.A Ty1 integrase nuclear localization signal required for retrotranspositionCorrect integration of model substrates by Ty1 integraseA Ty1 reverse transcriptase active-site aspartate mutation blocks transposition but not polymerizationIncreased length of long terminal repeats inhibits Ty1 transposition and leads to the formation of tandem multimers.The application of a homologous recombination assay revealed amino acid residues in an LTR-retrotransposon that were critical for integration.Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.The Ty1 LTR-retrotransposon of budding yeast, Saccharomyces cerevisiaeSubstrate specificity of Ty1 integrase.A critical proteolytic cleavage site near the C terminus of the yeast retrotransposon Ty1 Gag protein.Retrovirus-like end processing of the tobacco Tnt1 retrotransposon linear intermediates of replication.In vitro integration of retrotransposon Ty1: a direct physical assay.Hybrid Ty1/HIV-1 elements used to detect inhibitors and monitor the activity of HIV-1 reverse transcriptaseFunctional analysis of N-terminal residues of ty1 integrase.Cooperation between reverse transcriptase and integrase during reverse transcription and formation of the preintegrative complex of Ty1.Ty1 proteolytic cleavage sites are required for transposition: all sites are not created equal.Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA.Efficient integration of artificial transposons into plasmid targets in vitro: a useful tool for DNA mapping, sequencing and genetic analysis.Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: a fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase-RNase H domain exhibits polymerase and RNase H actiTy1 in vitro integration: effects of mutations in cis and in trans.Genes of the Pseudoviridae (Ty1/copia retrotransposons).
P2860
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P2860
Expression and partial purification of enzymatically active recombinant Ty1 integrase in Saccharomyces cerevisiae
description
1994 nî lūn-bûn
@nan
1994 թուականի Մարտին հրատարակուած գիտական յօդուած
@hyw
1994 թվականի մարտին հրատարակված գիտական հոդված
@hy
1994年の論文
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1994年論文
@yue
1994年論文
@zh-hant
1994年論文
@zh-hk
1994年論文
@zh-mo
1994年論文
@zh-tw
1994年论文
@wuu
name
Expression and partial purific ...... se in Saccharomyces cerevisiae
@ast
Expression and partial purific ...... se in Saccharomyces cerevisiae
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type
label
Expression and partial purific ...... se in Saccharomyces cerevisiae
@ast
Expression and partial purific ...... se in Saccharomyces cerevisiae
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prefLabel
Expression and partial purific ...... se in Saccharomyces cerevisiae
@ast
Expression and partial purific ...... se in Saccharomyces cerevisiae
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P2860
P356
P1476
Expression and partial purific ...... se in Saccharomyces cerevisiae
@en
P2093
D J Garfinkel
P2860
P304
P356
10.1073/PNAS.91.5.1843
P407
P577
1994-03-01T00:00:00Z