Protein engineering by cDNA recombination in yeasts: shuffling of mammalian cytochrome P-450 functions.
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Analysis of the role of the hypervariable region of yeast Ras2p and its farnesylation in the interaction with exchange factors and adenylyl cyclase.Connections between RNA splicing and DNA intron mobility in yeast mitochondria: RNA maturase and DNA endonuclease switching experimentsDomain B protruding at the third beta strand of the alpha/beta barrel in barley alpha-amylase confers distinct isozyme-specific properties.Growth factor engineering by degenerate homoduplex gene family recombination.High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2Efficient library construction by in vivo recombination with a telomere-originated autonomously replicating sequence of Hansenula polymorphaA genetic system for direct selection of gene-positive clones during recombinational cloning in yeastHomologous and homeologous intermolecular gene conversion are not differentially affected by mutations in the DNA damage or the mismatch repair genes RAD1, RAD50, RAD51, RAD52, RAD54, PMS1 and MSH2A Heritable Recombination system for synthetic Darwinian evolution in yeast.Engineered yeasts simulating P450-dependent metabolisms: tricks, myths and reality.Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.A female-biased expressed elongase involved in long-chain hydrocarbon biosynthesis and courtship behavior in Drosophila melanogasterIn-frame recombination between the yeast H(+)-ATPase isogenes PMA1 and PMA2: insights into the mechanism of recombination initiated by a double-strand break.Homologous, homeologous, and illegitimate repair of double-strand breaks during transformation of a wild-type strain and a rad52 mutant strain of Saccharomyces cerevisiae.Induction of recombination between homologous and diverged DNAs by double-strand gaps and breaks and role of mismatch repairChimeras of the human cytochrome P450 1A family produced in yeast. Accumulation in microsomal membranes, enzyme kinetics and stability.Optimization of yeast-expressed human liver cytochrome P450 3A4 catalytic activities by coexpressing NADPH-cytochrome P450 reductase and cytochrome b5.Expression in yeast of three allelic cDNAs coding for human liver P-450 3A4. Different stabilities, binding properties and catalytic activities of the yeast-produced enzymes.Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution.Positive autoregulation of the yeast transcription factor Pdr3p, which is involved in control of drug resistance.High-throughput functional screening of steroid substrates with wild-type and chimeric P450 enzymes.A simple and highly efficient procedure for rescuing autonomous plasmids from yeast.Cytochromes P450: their active-site structure and mechanism of oxidation.
P2860
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P2860
Protein engineering by cDNA recombination in yeasts: shuffling of mammalian cytochrome P-450 functions.
description
1989 nî lūn-bûn
@nan
1989年の論文
@ja
1989年論文
@yue
1989年論文
@zh-hant
1989年論文
@zh-hk
1989年論文
@zh-mo
1989年論文
@zh-tw
1989年论文
@wuu
1989年论文
@zh
1989年论文
@zh-cn
name
Protein engineering by cDNA re ...... an cytochrome P-450 functions.
@ast
Protein engineering by cDNA re ...... an cytochrome P-450 functions.
@en
type
label
Protein engineering by cDNA re ...... an cytochrome P-450 functions.
@ast
Protein engineering by cDNA re ...... an cytochrome P-450 functions.
@en
prefLabel
Protein engineering by cDNA re ...... an cytochrome P-450 functions.
@ast
Protein engineering by cDNA re ...... an cytochrome P-450 functions.
@en
P1433
P1476
Protein engineering by cDNA re ...... an cytochrome P-450 functions.
@en
P2093
P356
10.1016/0378-1119(89)90399-5
P407
P577
1989-11-01T00:00:00Z