Thermodynamic and kinetic methods of analyses of protein-nucleic acid interactions. From simpler to more complex systems.
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Analytical Ultracentrifugation as a Tool to Study Nonspecific Protein-DNA InteractionsOptimizing scoring function of protein-nucleic acid interactions with both affinity and specificity.Energetics of the Escherichia coli DnaT protein trimerization reaction.The Arg-62 residues of the TREX1 exonuclease act across the dimer interface contributing to catalysis in the opposing protomers.The Escherichia coli PriA helicase specifically recognizes gapped DNA substrates: effect of the two nucleotide-binding sites of the enzyme on the recognition process.UNC-45B chaperone: the role of its domains in the interaction with the myosin motor domain.Monomeric nature of dengue virus NS3 helicase and thermodynamic analysis of the interaction with single-stranded RNA.Unique properties of the Mtr4p-poly(A) complex suggest a role in substrate targeting.Escherichia coli DnaB helicase-DnaC protein complex: allosteric effects of the nucleotides on the nucleic acid binding and the kinetic mechanism of NTP hydrolysis. 3Dynamics of the ssDNA recognition by the RepA hexameric helicase of plasmid RSF1010: analyses using fluorescence stopped-flow intensity and anisotropy methods.Mechanism of NTP hydrolysis by the Escherichia coli primary replicative helicase DnaB protein. 2. Nucleotide and nucleic acid specificities.Mechanisms of interactions of the nucleotide cofactor with the RepA protein of plasmid RSF1010. Binding dynamics studied using the fluorescence stopped-flow methodInteractions of the Escherichia coli DnaB-DnaC protein complex with nucleotide cofactors. 1. Allosteric conformational transitions of the complex.The Escherichia coli PriA helicase-double-stranded DNA complex: location of the strong DNA-binding subsite on the helicase domain of the protein and the affinity control by the two nucleotide-binding sites of the enzyme.Full-length Dengue virus RNA-dependent RNA polymerase-RNA/DNA complexes: stoichiometries, intrinsic affinities, cooperativities, base, and conformational specificities.Evidence of Kinetic Cooperativity in Dimeric Ketopantoate Reductase from Staphylococcus aureus.Solution structures of 2 : 1 and 1 : 1 DNA polymerase-DNA complexes probed by ultracentrifugation and small-angle X-ray scattering.SSB-DNA binding monitored by fluorescence intensity and anisotropy.Macromolecular competition titration method accessing thermodynamics of the unmodified macromolecule-ligand interactions through spectroscopic titrations of fluorescent analogs.Interactions between the Dengue Virus Polymerase NS5 and Stem-Loop A.Hidden entropic contribution in the thermodynamics of molecular complexation.The N-terminal domain of the Escherichia coli PriA helicase contains both the DNA- and nucleotide-binding sites. Energetics of domain--DNA interactions and allosteric effect of the nucleotide cofactorsDetermination of equilibrium and rate constants for complex formation by fluorescence correlation spectroscopy supplemented by dynamic light scattering and Taylor dispersion analysis.Interactions of the Escherichia coli primosomal PriB protein with the single-stranded DNA. Stoichiometries, intrinsic affinities, cooperativities, and base specificities.Comparative analysis of interaction of human and yeast DNA damage recognition complexes with damaged DNA in nucleotide excision repair.Kinetic mechanism of the ssDNA recognition by the polymerase X from African Swine Fever Virus. Dynamics and energetics of intermediate formations.Interactions of the DNA polymerase X from African Swine Fever Virus with the ssDNA. Properties of the total DNA-binding site and the strong DNA-binding subsite.Thermodynamic analysis of the structure-function relationship in the total DNA-binding site of enzyme-DNA complexesCooperative DNA binding and communication across the dimer interface in the TREX2 3' --> 5'-exonucleaseThe Escherichia coli primosomal DnaT protein exists in solution as a monomer-trimer equilibrium systemQuantitative Thermodynamic Analyses of Spectroscopic Titration Curves.The primary DNA-binding subsite of the rat pol β. Energetics of interactions of the 8-kDa domain of the enzyme with the ssDNAFluorescence intensity, anisotropy, and transient dynamic quenching stopped-flow kinetics.Examination of polypeptide substrate specificity for Escherichia coli ClpB.Complete solution of the problem of one-dimensional non-covalent non-cooperative self-assembly in two-component systems.
P2860
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P2860
Thermodynamic and kinetic methods of analyses of protein-nucleic acid interactions. From simpler to more complex systems.
description
2006 nî lūn-bûn
@nan
2006年の論文
@ja
2006年学术文章
@wuu
2006年学术文章
@zh-cn
2006年学术文章
@zh-hans
2006年学术文章
@zh-my
2006年学术文章
@zh-sg
2006年學術文章
@yue
2006年學術文章
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2006年學術文章
@zh-hant
name
Thermodynamic and kinetic meth ...... mpler to more complex systems.
@ast
Thermodynamic and kinetic meth ...... mpler to more complex systems.
@en
type
label
Thermodynamic and kinetic meth ...... mpler to more complex systems.
@ast
Thermodynamic and kinetic meth ...... mpler to more complex systems.
@en
prefLabel
Thermodynamic and kinetic meth ...... mpler to more complex systems.
@ast
Thermodynamic and kinetic meth ...... mpler to more complex systems.
@en
P356
P1433
P1476
Thermodynamic and kinetic meth ...... mpler to more complex systems.
@en
P2093
Wlodzimierz Bujalowski
P304
P356
10.1021/CR040462L
P577
2006-02-01T00:00:00Z